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评估引物OPF - 01、P54和1253，以从RAPD - PCR获得的多态性模式中鉴定、和。 （原文中此处有缺失内容）

Evaluation of Primers OPF-01, P54, and 1253 to Identify , , and from Polymorphic Patterns Obtained by RAPD-PCR.

作者信息

Castro-Fuentes Carlos Alberto, Frías-De-León María Guadalupe, González-Villaseñor María Del Carmen, Duarte-Escalante Esperanza, Valencia-Ledezma Omar Esteban, Martínez-Gamboa Areli, Meraz-Ríos Beatriz, Reyes-Montes María Del Rocío

机构信息

Posgrado en Ciencias Biológicas, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.

Unidad de Investigación, Hospital Regional de Alta Especialidad de Ixtapaluca, IMSS-Bienestar. Calle Gustavo E. Campa 54, Col. Guadalupe Inn, Alcaldía Álvaro Obregón, Mexico City 01020, Mexico.

出版信息

Pathogens. 2024 Jul 10;13(7):574. doi: 10.3390/pathogens13070574.

DOI:10.3390/pathogens13070574
PMID:39057801
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11280055/
Abstract

We evaluated the specificity of the primers OPF-01, P54, and 1253 to identify , , and , respectively, with the RAPD-PCR method. Eighty-two isolates belonging to the sections , , and were used. The isolates were identified by phenotypic (macro- and micromorphology) and genotypic (partial sequences of the gene) methods. The RAPD-PCR method was used to obtain polymorphic patterns with the primers OPF-01, P54, and 1253. The specificity of the polymorphic patterns of the isolates of each species was evaluated through the UPGMA clustering method and logistic regression model. All isolates of the genus were identified at the section level by macro- and micromorphology showing the typical morphology of the sections , , and , and the species were identified by the construction of the phylogeny of the partial sequence of the gene. The patterns' polymorphic strains obtained with the primers OPF-01, P54, and 1253 for the isolates of , , and , respectively, showed the same polymorphic pattern as the reference strains for each species. To verify the specificity of the primers, they were tested with other species from the sections , and The results support that the primers OPF-01, P54, and 1253 generate polymorphic patterns by RAPD-PCR species specific to , , and , respectively.

摘要

我们使用RAPD-PCR方法评估了引物OPF-01、P54和1253分别鉴定[具体物种1]、[具体物种2]和[具体物种3]的特异性。使用了属于[相关分类1]、[相关分类2]和[相关分类3]组的82个分离株。通过表型（宏观和微观形态）和基因型（[基因名称]基因的部分序列）方法对分离株进行鉴定。使用RAPD-PCR方法，用引物OPF-01、P54和1253获得多态性图谱。通过UPGMA聚类方法和逻辑回归模型评估每个物种分离株多态性图谱的特异性。通过宏观和微观形态在组水平鉴定了[属名]属的所有分离株，显示出[相关分类1]、[相关分类2]和[相关分类3]组的典型形态，并通过构建[基因名称]基因部分序列的系统发育树鉴定了物种。分别用引物OPF-01、P54和1253为[具体物种1]、[具体物种2]和[具体物种3]的分离株获得的多态性菌株图谱与每个物种的参考菌株显示出相同的多态性图谱。为验证引物的特异性，用来自[相关分类1]、[相关分类2]和[相关分类3]组的其他物种对其进行了测试。结果支持引物OPF-01、P54和1253通过RAPD-PCR分别产生特定于[具体物种1]、[具体物种2]和[具体物种3]的多态性图谱。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/2f08de568b25/pathogens-13-00574-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/3e869d4261ea/pathogens-13-00574-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/4e4a429791f4/pathogens-13-00574-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/66576215a6a4/pathogens-13-00574-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/e1b469f0a642/pathogens-13-00574-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/285bc659b028/pathogens-13-00574-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/b5c0503bcd5f/pathogens-13-00574-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/b85c1dc45824/pathogens-13-00574-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/93c595c04c3b/pathogens-13-00574-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/2f08de568b25/pathogens-13-00574-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/3e869d4261ea/pathogens-13-00574-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/4e4a429791f4/pathogens-13-00574-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/66576215a6a4/pathogens-13-00574-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/e1b469f0a642/pathogens-13-00574-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/285bc659b028/pathogens-13-00574-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/b5c0503bcd5f/pathogens-13-00574-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/b85c1dc45824/pathogens-13-00574-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/93c595c04c3b/pathogens-13-00574-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d967/11280055/2f08de568b25/pathogens-13-00574-g009.jpg

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