Koziy Roman V, Bracamonte José L, Katselis George S, Udenze Daniel, Hayat Shahina, Hammond S Austin, Simko Elemir
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Vet Sci. 2024 Jul 2;11(7):299. doi: 10.3390/vetsci11070299.
Septic arthritis (SA) in horses has long-term health implications. The success of its resolution hinges on the implementation of early, aggressive treatment, which is often sustained over a prolonged period. Common diagnostic methods do not allow for the reliable detection of the eradication of joint infection. A potential alternative is the discovery and characterization of mRNA biomarkers. The purpose of this study was to identify potential mRNA biomarkers for the eradication of joint infection in equine SA and to compare their expression with our previously published proteomics data. In addition, the transcriptomics data were compared to the mRNA biomarker panel, SeptiCyte Lab, used to distinguish sepsis from non-septic shock in humans. A comparative transcriptomics analysis of synovial fluid from the SA joints of five horses with active infection and subsequent post-treatment eradicated infection in the same joints and five horses with non-septic synovitis was performed. Eight novel mRNA transcripts were identified that were significantly upregulated (>3-fold) in horses with active SA compared to horses post-eradication of infection after treatment and horses with non-septic synovitis. Two proteins in our proteomics data corresponded to these mRNA transcripts, but were not statistically different. The transcripts used in the SeptiCyte test were not differentially expressed in our study. Our results suggest that mRNA may be a useful source of biomarkers for the eradication of joint infection in horses and warrants further investigation.
马的脓毒性关节炎（SA）具有长期健康影响。其病情缓解的成功取决于早期积极治疗的实施，这种治疗通常要持续较长时间。常用的诊断方法无法可靠地检测出关节感染是否已消除。一种潜在的替代方法是发现和鉴定mRNA生物标志物。本研究的目的是确定马SA中消除关节感染的潜在mRNA生物标志物，并将它们的表达与我们之前发表的蛋白质组学数据进行比较。此外，还将转录组学数据与用于区分人类脓毒症和非脓毒性休克的mRNA生物标志物检测板SeptiCyte Lab进行了比较。对五匹患有活动性感染的马的SA关节滑液以及同一关节后续治疗后感染已消除的滑液，和五匹患有非脓毒性滑膜炎的马的滑液进行了比较转录组学分析。鉴定出八个新的mRNA转录本，与治疗后感染已消除的马以及患有非脓毒性滑膜炎的马相比，这些转录本在患有活动性SA的马中显著上调（>3倍）。我们蛋白质组学数据中的两种蛋白质与这些mRNA转录本相对应，但无统计学差异。SeptiCyte检测中使用的转录本在我们的研究中没有差异表达。我们的结果表明，mRNA可能是用于鉴定马关节感染是否已消除的生物标志物的有用来源，值得进一步研究。
