Koziy Roman V, Yoshimura Seiji, Dickinson Ryan, Rybicka Joanna M, Moshynskyy Igor, Ngeleka Musangu, Bracamonte Jose L, Simko Elemir
Department of Veterinary Pathology (Koziy, Dickinson, Rybicka, Moshynskyy, Simko), Department of Large Animal Clinical Sciences (Yoshimura, Bracamonte), and Prairie Diagnostic Services Inc. (Ngeleka), Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4.
Can J Vet Res. 2019 Jan;83(1):24-33.
Septic arthritis is an important disease in horses, necessitating aggressive and prolonged therapy. In order to guide therapy, reliable methods of detecting the eradication of infection are needed. Therefore, the objective of this study was to investigate detection of eradication of infection in an experimental model of equine septic arthritis using standard diagnostic techniques. For this purpose, 17 adult horses were assigned to 3 experimental groups. The middle carpal joint of each horse was injected with (Septic group, = 8), lipopolysaccharide (LPS) (LPS group, = 6), or sterile saline (Control group, = 3) at day 0. Contralateral joints were not injected. Standard therapy was applied to all joints except non-injected joints in the Control group at day 1. Sequential samples of synovial fluid (SF) were collected for bacterial culture using 3 culture media [Columbia blood agar (CBA), brain heart infusion broth (BHI), and Signal blood culture medium] and for cytological evaluation [percentage neutrophils (PN), total nucleated cell count (TNCC), and total protein (TP)]. -specific polymerase chain reaction (PCR) was carried out to detect DNA in synovial fluid. Culture and PCR were positive for in all joints injected with at day 1 and 1 joint was positive on BHI at day 4. Based on the results of bacterial culture, PCR, and TNCC, the elimination of infection in our experimental model occurred by day 4 post-infection in 6 out of 7 cases. Total protein (TP) and PN remained elevated at clinical threshold used for diagnosis of septic arthritis until day 14. In our experimental model of -induced arthritis, we conclude that TP and PN may not be good indicators for detecting the eradication of bacterial infection caused by from infected and subsequently treated joints.
脓毒性关节炎是马匹的一种重要疾病，需要积极且长期的治疗。为了指导治疗，需要可靠的方法来检测感染的根除情况。因此，本研究的目的是使用标准诊断技术，在马脓毒性关节炎的实验模型中研究感染根除的检测方法。为此，将17匹成年马分为3个实验组。在第0天，给每匹马的腕中关节注射（脓毒症组， = 8）、脂多糖（LPS）（LPS组， = 6）或无菌生理盐水（对照组， = 3）。对侧关节不进行注射。除对照组未注射的关节外，所有关节在第1天均采用标准治疗。使用3种培养基[哥伦比亚血琼脂（CBA）、脑心浸液肉汤（BHI）和信号血培养基]收集滑膜液（SF）的连续样本进行细菌培养，并进行细胞学评估[中性粒细胞百分比（PN）、总核细胞计数（TNCC）和总蛋白（TP）]。进行特异性聚合酶链反应（PCR）以检测滑膜液中的DNA。在第1天，所有注射的关节培养和PCR均为阳性，在第4天，1个关节在BHI上呈阳性。根据细菌培养、PCR和TNCC的结果，在7例中的6例中，感染在感染后第4天在我们的实验模型中被清除。总蛋白（TP）和PN在用于诊断脓毒性关节炎的临床阈值下一直升高到第14天。在我们诱导的关节炎实验模型中，我们得出结论，TP和PN可能不是检测由感染并随后治疗的关节中引起的细菌感染根除的良好指标。
