Hanifeh Mohsen, Huhtinen Mirja, Sclivagnotis Yannes S, Lyhs Ulrike, Grönthal Thomas, Spillmann Thomas
Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Viikintie 49, 00014 Helsinki, Finland.
Orion Corporation, Orion Pharma, R&D, Orionintie 1A, 02200 Espoo, Finland.
Vet Sci. 2024 Jul 16;11(7):319. doi: 10.3390/vetsci11070319.
The relative abundances of Bacteroidetes and Fusobacteria phyla have been reported to be decreased in dogs with chronic enteropathies. In colitis, obligate anaerobes (e.g., and ) are likely to vanish in response to the heightened oxidative stress in the colon's inflammatory environment. The ability to adhere to the colonic mucosa is viewed as an essential step for obligate anaerobic bacteria to colonize and subsequently interact with the host's epithelium and immune system. The reintroduction of a balanced community of obligate anaerobic bacteria using probiotics can restore the microbial function in the intestine. We found no studies on dogs regarding the adhesion properties of and on paraffin-embedded canine colonic mucosa. Thus, the objective of this study is to investigate the adhesion capacities of these two bacterial species to paraffin-embedded colonic mucosa from healthy dogs. Additionally, we investigated their hydrophobicity properties to determine whether differences in adhesion capability can be explained by this factor. The results of our study showed that adhered significantly lower than to the canine colonic mucosa ( = 0.002); however, showed higher hydrophobicity (46.1%) than (12.6%). In conclusion, both bacteria have potential as probiotics, but further studies will be required to determine the efficacy and safety of the strains to be used, which strains to use, and the reasons other than hydrophobicity for attachment.
据报道，患有慢性肠病的犬类中拟杆菌门和梭杆菌门的相对丰度会降低。在结肠炎中，专性厌氧菌（如 和 ）可能会因结肠炎症环境中氧化应激加剧而消失。黏附于结肠黏膜的能力被视为专性厌氧菌定殖并随后与宿主上皮和免疫系统相互作用的关键步骤。使用益生菌重新引入平衡的专性厌氧菌群落可以恢复肠道中的微生物功能。我们未发现有关 和 对石蜡包埋的犬结肠黏膜黏附特性的犬类研究。因此，本研究的目的是调查这两种细菌对健康犬石蜡包埋结肠黏膜的黏附能力。此外，我们研究了它们的疏水性，以确定黏附能力的差异是否可以用该因素来解释。我们的研究结果表明， 对犬结肠黏膜的黏附明显低于 （ = 0.002）；然而， 的疏水性（46.1%）高于 （12.6%）。总之，这两种细菌都有作为益生菌的潜力，但需要进一步研究以确定所用菌株的功效和安全性、使用哪些菌株以及除疏水性外的其他附着原因。
