Division of Cancer Imaging Research, The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Department of Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Tomography. 2024 Jul 13;10(7):1099-1112. doi: 10.3390/tomography10070083.
: The standard of care for stage 1 NSCLC is upfront surgery followed by surveillance. However, 20-30% of stage 1 NSCLC recur. There is an unmet need to identify individuals likely to recur who would benefit from frequent monitoring and aggressive cancer treatments. Collagen 1 (Col1) fibers detected by second harmonic generation (SHG) microscopy are a major structural component of the extracellular matrix (ECM) of tumors that play a role in cancer progression. : We characterized Col1 fibers with SHG microscopy imaging of surgically resected stage 1 NSCLC. Gene expression from RNA sequencing data was used to validate the SHG microscopy findings. : We identified a significant ( ≤ 0.05) increase in the Col1 fiber volume in stage 1 NSCLC that recurred. The increase in Col1 fiber volume was supported by significant increases in the gene expression of Col1 in invasive, compared to noninvasive, lung adenocarcinoma. Significant differences were identified in the gene expression of other ECM proteins, as well as CAFs, immune checkpoint markers, immune cytokines, and T-cell markers. Col1 fiber analysis can provide a companion diagnostic test to evaluate the likelihood of tumor recurrence following stage 1 NSCLC. The studies expand our understanding of the role of the ECM in NSCLC recurrence.
: Ⅰ期非小细胞肺癌的标准治疗方法是手术切除后进行监测。然而，仍有 20-30%的Ⅰ期非小细胞肺癌患者会复发。因此，需要识别出那些可能会复发的个体，以便他们能从频繁监测和积极的癌症治疗中受益。通过二次谐波（SHG）显微镜检测到的胶原 1 （Col1）纤维是肿瘤细胞外基质（ECM）的主要结构成分，在癌症进展中发挥作用。 : 我们通过对手术切除的Ⅰ期非小细胞肺癌进行 SHG 显微镜成像来描述 Col1 纤维。使用 RNA 测序数据的基因表达来验证 SHG 显微镜的发现。 : 我们发现，复发的Ⅰ期非小细胞肺癌中 Col1 纤维体积显著增加（≤0.05）。与非侵袭性肺腺癌相比，侵袭性肺腺癌中 Col1 基因表达显著增加，这支持了 Col1 纤维体积增加的结论。其他 ECM 蛋白、CAFs、免疫检查点标志物、免疫细胞因子和 T 细胞标志物的基因表达也存在显著差异。 Col1 纤维分析可提供一种伴随诊断测试，用于评估Ⅰ期非小细胞肺癌患者术后肿瘤复发的可能性。这些研究扩展了我们对 ECM 在非小细胞肺癌复发中的作用的理解。
