Leoni S, Spagnuolo S, Conti Devirgiliis L, Dini L, Mangiantini M T, Trentalance A
J Cell Physiol. 1985 Dec;125(3):507-11. doi: 10.1002/jcp.1041250321.
The effect of glucagon and insulin on the incorporation of 1-14C-acetate into cholesterol and fatty acids and on the enzymes involved in the first steps of cholesterol synthesis (3-hydroxy-3-methyl-glutaryl-coenzyme A reductase, 3-hydroxy-3-methyl-glutaryl-coenzyme A synthase, and acetoacetyl-coenzyme A thiolase) was investigated. Isolated rat hepatocytes at different stages of fetal and postnatal development were employed. Data obtained show the appearance of hormonal control on the 18th day of fetal life, indicating the same pattern, as regards acetate incorporation and HMGCoA reductase prepared and assayed in the presence of NaF. On the contrary, HMGCoA reductase, prepared without NaF, HMGCoA synthase, and acetoacetyl CoA thiolase, does not appear to respond to hormonal stimulation. In the perinatal period, the hormonal effect is no longer detectable, probably because of a hormone resistance of this metabolic pathway.
研究了胰高血糖素和胰岛素对1-¹⁴C-乙酸掺入胆固醇和脂肪酸以及对胆固醇合成第一步所涉及的酶（3-羟基-3-甲基戊二酰辅酶A还原酶、3-羟基-3-甲基戊二酰辅酶A合酶和乙酰乙酰辅酶A硫解酶）的影响。使用了处于胎儿和出生后不同发育阶段的分离大鼠肝细胞。所获得的数据显示在胎儿期第18天出现激素控制，就乙酸掺入以及在氟化钠存在下制备和测定的HMGCoA还原酶而言，呈现相同模式。相反，在没有氟化钠的情况下制备的HMGCoA还原酶、HMGCoA合酶和乙酰乙酰辅酶A硫解酶似乎对激素刺激没有反应。在围产期，激素效应不再可检测到，可能是因为该代谢途径存在激素抵抗。
