Leoni S, Spagnuolo S, Conti-Devirgiliis L, Dini L, Mangiantini M T, Trentalance A
J Cell Physiol. 1984 Jan;118(1):62-6. doi: 10.1002/jcp.1041180112.
Cholesterogenesis pathway during pre- and postnatal development was studied in isolated rat hepatocytes. No modified activity of cytosol acetoacetyl coenzyme A (CoA), thiolase, or 3-hydroxy-3-methylglutaryl CoA (HMGCoA) synthase was detectable at the different stages examined. Minimal levels of 1(14)C-acetate incorporation into cholesterol and HMGCoA reductase activity were present at 16 days of fetal development in newborn and suckling rats, whereas both parameters increased rapidly before birth. The pattern of NaF nonsuppressible reductase activity showed a different activation state of the enzyme, suggesting the appearance of a modulation state, probably related to the development of some short-term regulatory mechanisms.
在分离的大鼠肝细胞中研究了产前和产后发育期间的胆固醇生物合成途径。在所检测的不同阶段，均未检测到胞质乙酰乙酰辅酶A（CoA）、硫解酶或3-羟基-3-甲基戊二酰辅酶A（HMGCoA）合酶的活性改变。在新生和哺乳大鼠的胎儿发育第16天时，1(14)C-乙酸掺入胆固醇和HMGCoA还原酶活性的水平最低，而这两个参数在出生前迅速增加。NaF不可抑制的还原酶活性模式显示该酶处于不同的激活状态，表明出现了一种调节状态，可能与某些短期调节机制的发育有关。
