Bio-Medical Engineering Group, Drug Discovery Laboratory, Teijin Institute for Bio-Medical Research, Teijin Pharma Ltd., Tokyo, Japan.
Department of Neurology, School of Medicine, International University of Health and Welfare, Chiba, Japan.
PLoS One. 2024 Jul 26;19(7):e0306527. doi: 10.1371/journal.pone.0306527. eCollection 2024.
Photobiomodulation selectively controls the activity of the sensory nervous system associated with A-delta and C fibers. Hypersensitivity involving the afferent A-delta and C fibers occurs in cystitis and decreases urinary function. This study aimed to investigate the effect of photobiomodulation on urinary storage dysfunction and voiding functions in cystitis model rats.
We prepared the rat cystitis model. Under anesthesia, a cannula was connected to the bladder via a ventral incision. 0.3% acetic acid or saline was injected into the bladder. Continuous cystometry was performed, measuring bladder pressure and voiding urine volume with rats freely mobile. Laser irradiation was applied to the L6 lumbosacral intervertebral foramen using an 830 nm laser. Residual urine was extracted post-cystometry.
In the rat cystitis model groups, there was a significant decrease in the voiding interval and volume compared to the group receiving normal saline infusion. After sham or laser irradiation, only the group with laser irradiation showed a significant increase in voiding interval (217%, p = 0.0002) and voiding volume (192%, p = 0.0012) in the parameters of storage dysfunction. The basal pressure, intravesical pressure, and residual urine volume remained unchanged in all groups before and after irradiation.
This study indicates that photobiomodulation may improve urine storage dysfunction without exacerbating voiding function in a rat model of cystitis. Thus, photobiomodulation may be a new treatment option for the hypersensitivity and detrusor overactivity caused by cystitis.
光生物调节选择性地控制与 A-δ 和 C 纤维相关的感觉神经系统的活动。涉及传入 A-δ 和 C 纤维的过敏反应在膀胱炎中发生，并降低尿功能。本研究旨在探讨光生物调节对膀胱炎模型大鼠储尿功能和排尿功能的影响。
我们制备了大鼠膀胱炎模型。在麻醉下，通过腹侧切口将导管连接到膀胱。向膀胱内注射 0.3%乙酸或生理盐水。使用 830nm 激光对 L6 腰骶椎间孔进行激光照射。连续膀胱测压，使大鼠自由移动，测量膀胱压力和排尿量。
在大鼠膀胱炎模型组中，与生理盐水输注组相比，排尿间隔和排尿量明显减少。假照射或激光照射后，只有激光照射组在储尿功能参数中显示出明显的排尿间隔（217%，p=0.0002）和排尿量（192%，p=0.0012）增加。照射前后所有组的基础压、膀胱内压和残余尿量均保持不变。
本研究表明，光生物调节可能改善膀胱炎大鼠的储尿功能障碍，而不会加重排尿功能。因此，光生物调节可能成为治疗膀胱炎引起的过敏和逼尿肌过度活动的新治疗选择。
