Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
Key Laboratory of Ocular Fundus Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People's Republic of China.
Biomed Mater. 2024 Aug 8;19(5). doi: 10.1088/1748-605X/ad6826.
To explore the feasibility and safety of biomaterials for posterior scleral reinforcement (PSR) in rabbits. Decellularization and genipin crosslink were applied to the fresh bovine pericardium and porcine endocranium, and then mechanical properties, suture retention strength, and stability were tested. PSR operation was performed on 24 rabbit eyes using treated biological materials. Ophthalmic examination was performed regularly before and after PSR operation (1 week, 1 month, 3 months, 6 months). To evaluate the effectiveness, A ultrasound, diopter, and optical coherence tomography were conducted. General condition, fundus photograph, and pathological examination were recorded to evaluate the safety. Compared with genipin crosslinked bovine pericardium (Gen-BP) (21.29 ± 13.29 Mpa), genipin crosslinked porcine endocranium (Gen-PE) (34.85 ± 3.67 Mpa,< 0.01) showed a closer elastic modulus to that of genipin crosslinked human sclera. There were no complications or toxic reactions directly related to the materials. Capillary hyperplasia, inflammatory cell infiltration, and collagen fiber deposition were observed, and the content of type I collagen fibers increased after PSR. Overall, the choroidal thickness of treated eyes was significantly thickened at different time points after PSR, which were 96.84 ± 21.08 μm, 96.72 ± 22.00 μm, 90.90 ± 16.57 μm, 97.28 ± 14.74 μm, respectively. The Gen-PE group showed changes that were almost consistent with the overall data. Gen-BP and Gen-PE are safe biological materials for PSR. The Gen-PE group demonstrated more significant advantages over the Gen-BP group in terms of material properties.
探讨生物材料用于兔眼后巩膜加固术（PSR）的可行性和安全性。对新鲜牛心包和猪颅骨进行脱细胞和京尼平交联处理，然后测试其力学性能、缝线保持强度和稳定性。用处理后的生物材料对 24 只兔眼进行 PSR 手术。PSR 前后（1 周、1 个月、3 个月、6 个月）定期行眼科检查。为评估疗效，进行 A 超、屈光度和光学相干断层扫描。记录一般情况、眼底照相和病理检查以评估安全性。与京尼平交联牛心包（Gen-BP）（21.29±13.29Mpa）相比，京尼平交联猪颅骨（Gen-PE）（34.85±3.67Mpa，<0.01）具有更接近的弹性模量与人巩膜交联的 Gen-PE。没有直接与材料相关的并发症或毒性反应。观察到毛细血管增生、炎性细胞浸润和胶原纤维沉积，PSR 后 I 型胶原纤维含量增加。总的来说，PSR 后不同时间点处理眼的脉络膜厚度均显著增厚，分别为 96.84±21.08μm、96.72±22.00μm、90.90±16.57μm、97.28±14.74μm。Gen-PE 组的变化与总体数据基本一致。Gen-BP 和 Gen-PE 是 PSR 的安全生物材料。Gen-PE 组在材料性能方面较 Gen-BP 组具有更显著的优势。
