Huang Yu-Yan, Zhou Li-Yang, Chen Guo-Fu, Peng Duo, Pan Miao-Zhen, Zhou Ji-Bo, Qu Jia
National Clinical Research Center for Ocular Diseases, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, Zhejiang Province, China.
Hefei Aier Eye Hospital, Hefei 230000, Anhui Province, China.
Int J Ophthalmol. 2025 Mar 18;18(3):375-382. doi: 10.18240/ijo.2025.03.01. eCollection 2025.
To investigate the refractive and the histological changes in guinea pig eyes after posterior scleral reinforcement with scleral allografts.
Four-week-old guinea pigs were implanted with scleral allografts, and the changes of refraction, corneal curvature and axis length were monitored for 51d. The effects of methylprednisolone (MPS) on refraction parameters were also evaluated. And the microstructure and ultra-microstructure of eyes were observed on the 9d and 51d after operation. Repeated-measures analysis of variance and one-way analysis of variance were used.
The refraction outcome of the implanted eye decreased after operation, and the refraction change of the 3 mm scleral allografts group was significantly different with control group (=0.005) and the sham surgical group (=0.004). After the application of MPS solution, the reduction of refraction outcome was statistically suppressed (=0.008). The inflammatory encapsulation appeared 9d after surgery. On 51d after operation, the loose implanted materials were absorbed, while the adherent implanted materials with MPS group were still tightly attached to the recipient's eyeball.
After implantation of scleral allografts, the refraction of guinea pig eyes fluctuated from a decrease to an increase. The outcome of the scleral allografts is affected by implantation methods and the inflammatory response. Stability of the material can be improved by MPS.
研究同种异体巩膜后巩膜加固术对豚鼠眼屈光及组织学的影响。
对4周龄豚鼠行同种异体巩膜植入术，监测51天内屈光、角膜曲率及眼轴长度的变化。评估甲基强的松龙（MPS）对屈光参数的影响。分别于术后9天和51天观察眼部的微观结构和超微结构。采用重复测量方差分析和单因素方差分析。
术后植入眼的屈光结果下降，3mm同种异体巩膜组的屈光变化与对照组（P = 0.005）和假手术组（P = 0.004）有显著差异。应用MPS溶液后，屈光结果的下降得到统计学抑制（P = 0.008）。术后9天出现炎性包囊。术后51天，疏松的植入材料被吸收，而MPS组粘连的植入材料仍紧密附着于受体眼球。
同种异体巩膜植入后，豚鼠眼的屈光先下降后上升。同种异体巩膜的效果受植入方式和炎症反应的影响。MPS可提高材料的稳定性。
