State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, No.1299, Sansha Road, Qingdao, Shandong Province, 266404, PR China.
State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, No.1299, Sansha Road, Qingdao, Shandong Province, 266404, PR China; Laboratory for Marine Drugs and Bioproducts, Qingdao Marine Science and Technology Center, Qingdao, Shandong Province, 266237, PR China; Sanya Oceanographic Institution, Ocean University of China, Sanya, Hainan Province, 572024, PR China; Qingdao Institute of Marine Bioresources for Nutrition & Health Innovation, Qingdao, Shandong Province, 266000, PR China.
Food Res Int. 2024 Sep;191:114697. doi: 10.1016/j.foodres.2024.114697. Epub 2024 Jun 28.
The promoting effects of collagen and its derivatives on bone health have been uncovered. However, the structure and effects of type II collagen peptides from squid cartilage (SCIIP) on osteoarthritis still need to be clarified. In this study, SCIIP was prepared from squid throat cartilage with pretreatment by 0.2 mol/L NaOH at a liquid-solid ratio of 10:1 for 18 h and hydrolyzation using alkaline protease and flavourzyme at 50 °C for 4 h. The structure of SCIIP was characterized as a molecular weight lower than 5 kDa (accounting for 87.7 %), a high glycine level of 35.0 %, typical FTIR and CD features of collagen peptides, and a repetitive sequence of Gly-X-Y. GP(Hyp)GPD and GPAGP(Hyp)GD were separated and identified from SCIIP, and their binding energies with TLR4/MD-2 were - 8.4 and - 8.0 kcal/mol, respectively. SCIIP effectively inhibited NO production in RAW264.7 macrophages and alleviated osteoarthritis in rats through the TLR4/NF-κB pathway. Therefore, SCIIP exhibited the potential for application as an anti-osteoarthritis supplement.
胶原蛋白及其衍生物促进骨骼健康的作用已被揭示。然而，鱿鱼软骨（SCIIP）中 II 型胶原蛋白肽的结构和作用仍需要阐明。在这项研究中，鱿鱼喉软骨先用 0.2 mol/L NaOH 在液固比为 10:1 的条件下预处理 18 h，然后用碱性蛋白酶和风味蛋白酶在 50°C 下水解 4 h，制备得到 SCIIP。SCIIP 的结构特征为分子量低于 5 kDa（占 87.7%）、甘氨酸含量高（35.0%）、典型的胶原肽傅里叶变换红外光谱和圆二色性特征、以及 Gly-X-Y 重复序列。从 SCIIP 中分离并鉴定出 GP(Hyp)GPD 和 GPAGP(Hyp)GD，它们与 TLR4/MD-2 的结合能分别为-8.4 和-8.0 kcal/mol。SCIIP 通过 TLR4/NF-κB 通路有效抑制 RAW264.7 巨噬细胞中 NO 的产生，并减轻大鼠的骨关节炎。因此，SCIIP 具有作为抗骨关节炎补充剂的应用潜力。
