Sinden R E, Hartley R H
J Protozool. 1985 Nov;32(4):742-4. doi: 10.1111/j.1550-7408.1985.tb03113.x.
Zygotes of Plasmodium berghei were cultured 15-25 h in vitro to yield mature infective ookinetes. Samples taken in the first 5 h of culture were examined by electron microscopy. Meiotic figures were detected in the nuclei of the zygotes. Threadlike leptotene chromatids (chromosomes) condensed from attachment plaques on the nuclear envelope; chromatid pairing followed (zygotene), with synaptonemal complexes subsequently appearing (pachytene). These complexes persisted into metaphase but dissociated when the chromatids rapidly decondensed during anaphase. At telophase of the first meiotic division the kinetochores were retracted toward two small spindle complexes, which were found at widely separated poles in the nuclear envelope. The observations are consistent with a haploid genome of 8-10 chromosomes.
将伯氏疟原虫合子在体外培养15 - 25小时以产生成熟的感染性动合子。在培养的前5小时采集的样本通过电子显微镜检查。在合子核中检测到减数分裂图像。丝状细线期染色单体（染色体）从核膜上的附着斑浓缩形成；随后染色单体配对（偶线期），接着出现联会复合体（粗线期）。这些复合体持续到中期，但在后期染色单体迅速解聚时解离。在第一次减数分裂的末期，动粒向两个小纺锤体复合体回缩，这两个复合体位于核膜中相距很远的两极。这些观察结果与8 - 10条染色体的单倍体基因组一致。
