Charalampopoulou Alexandra, Barcellini Amelia, Peloso Andrea, Vanoli Alessandro, Cesari Stefania, Icaro Cornaglia Antonia, Bistika Margarita, Croce Stefania, Cobianchi Lorenzo, Ivaldi Giovanni Battista, Locati Laura Deborah, Magro Giuseppe, Tabarelli de Fatis Paola, Pullia Marco Giuseppe, Orlandi Ester, Facoetti Angelica
CNAO National Center for Oncological Hadrontherapy, Radiobiology Unit, Research and Development Department, 27100 Pavia, Italy.
Hadron Academy PhD Course, School for Advanced Studies (IUSS), 27100 Pavia, Italy.
Cancers (Basel). 2024 Jul 18;16(14):2582. doi: 10.3390/cancers16142582.
Decellularized extracellular matrix (ECM) bioscaffolds have emerged as a promising three-dimensional (3D) model, but so far there are no data concerning their use in radiobiological studies.
We seeded two well-known radioresistant cell lines (HMV-II and PANC-1) in decellularized porcine liver-derived scaffolds and irradiated them with both high- (Carbon Ions) and low- (Photons) Linear Energy Transfer (LET) radiation in order to test whether a natural 3D-bioscaffold might be a useful tool for radiobiological research and to achieve an evaluation that could be as near as possible to what happens in vivo.
Biological scaffolds provided a favorable 3D environment for cell proliferation and expansion. Cells did not show signs of dedifferentiation and retained their distinct phenotype coherently with their anatomopathological and clinical behaviors. The radiobiological response to high LET was higher for HMV-II and PANC-1 compared to the low LET. In particular, Carbon Ions reduced the melanogenesis in HMV-II and induced more cytopathic effects and the substantial cell deterioration of both cell lines compared to photons.
In addition to offering a suitable 3D model for radiobiological research and an appropriate setting for preclinical oncological analysis, we can attest that bioscaffolds seemed cost-effective due to their ease of use, low maintenance requirements, and lack of complex technology.
去细胞化细胞外基质（ECM）生物支架已成为一种有前景的三维（3D）模型，但到目前为止，尚无关于其在放射生物学研究中应用的数据。
我们将两种著名的抗辐射细胞系（HMV-II和PANC-1）接种到去细胞化猪肝脏衍生的支架中，并用高（碳离子）和低（光子）线性能量传递（LET）辐射对其进行照射，以测试天然3D生物支架是否可能成为放射生物学研究的有用工具，并获得尽可能接近体内情况的评估。
生物支架为细胞增殖和扩增提供了有利的3D环境。细胞未显示去分化迹象，并与其解剖病理学和临床行为一致地保留了其独特的表型。与低LET相比，HMV-II和PANC-1对高LET的放射生物学反应更高。特别是，与光子相比，碳离子减少了HMV-II中的黑色素生成，并诱导了更多的细胞病变效应以及两种细胞系的大量细胞退化。
除了为放射生物学研究提供合适的3D模型和为临床前肿瘤学分析提供适当的环境外，我们可以证明生物支架因其易于使用、低维护要求和缺乏复杂技术而似乎具有成本效益。
