Marchand R P, Mnzava A E
J Trop Med Hyg. 1985 Jun;88(3):205-10.
The usefulness of a proposed biochemical key to the species of the Anopheles gambiae complex is tested against the standard chromosomal method in N.E. Tanzania. The chromosomal identifications indicate the presence of three sibling species of the complex in this area, partly in coexisting populations. The electromorph frequencies at the diagnostic enzyme loci, octanol-dehydrogenase (Odh) and superoxide dismutase (Sod), show neither significant geographical nor seasonal variation. It is confirmed that A. merus can be distinguished unambiguously on its Sod genotype. A. gambiae s.s. and A. arabiensis can be identified, using the differences in their Odh polymorphism, with an average error of misidentification of 1 in 300. It is concluded that the biochemical key is sufficiently reliable to be a valuable additional taxonomic tool in the study area.
在坦桑尼亚东北部，一种针对冈比亚按蚊复合体物种的生化鉴定方法被用于与标准染色体方法进行对比测试。染色体鉴定表明该地区存在该复合体的三个姐妹种，部分存在于共存种群中。诊断酶位点辛醇脱氢酶（Odh）和超氧化物歧化酶（Sod）的电形态频率在地理和季节上均无显著变化。已证实，梅氏按蚊可根据其Sod基因型明确区分。利用冈比亚按蚊指名亚种和阿拉伯按蚊Odh多态性的差异可以进行鉴定，平均误判率为1/300。结论是，该生化鉴定方法足够可靠，可成为研究区域内有价值的额外分类工具。
