Van Rensburg A J, Hunt R H, Koekemoer L L, Coetzee M, Shiff C J, Minjas J
Department of Tropical Diseases, School of Pathology, South African Institute for Medical Research, Johannesburg, South Africa.
J Am Mosq Control Assoc. 1996 Jun;12(2 Pt 1):271-4.
Polymerase chain reaction (PCR) primers developed at the Centers for Disease Control in Atlanta for the identification of members of the Anopheles (Cellia) gambiae Giles complex were tested on material collected in the Bagamoyo and Muheza districts of northeastern Tanzania. Part of the sample from Bagamoyo was chromosomally identified and correlated with the PCR identifications. This sample contained 170 Anopheles arabiensis, 328 An. gambiae, and 58 Anopheles merus, of which 121, 237, and 54 specimens, respectively, were identified with both PCR and chromosomes. Three specimens identified chromosomally as An. merus gave only the PCR fragment characteristic for Anopheles quadriannulatus, but on retesting gave the correct result. The Muheza sample consisted of 771 An. arabiensis, 852 An. gambiae, 43 An. merus, and 4 specimens producing the fragment characteristic for An. quadriannulatus. Because An. quadriannulatus has never been recorded from mainland Tanzania and due to the high number of specimens that produced no result (193), it is probable that DNA degradation led to misidentification of An. merus specimens as An. quadriannulatus. The overall probability of correct identification by PCR was 99.685% at first testing, which compares favorably with other genetic methods currently in use.
亚特兰大疾病控制中心开发的用于鉴定冈比亚按蚊（Cellia）复合体成员的聚合酶链反应（PCR）引物，在坦桑尼亚东北部巴加莫约和穆赫扎地区采集的样本上进行了测试。巴加莫约样本的一部分经过染色体鉴定，并与PCR鉴定结果进行了关联。该样本包含170只阿拉伯按蚊、328只冈比亚按蚊和58只梅氏按蚊，其中分别有121只、237只和54只标本通过PCR和染色体鉴定均被识别出来。三只经染色体鉴定为梅氏按蚊的标本，最初PCR检测仅得到四带按蚊的特征片段，但重新检测后得到了正确结果。穆赫扎样本包括771只阿拉伯按蚊、852只冈比亚按蚊、43只梅氏按蚊以及4只产生四带按蚊特征片段的标本。由于在坦桑尼亚大陆从未记录到四带按蚊，且未得到结果的标本数量众多（193只），很可能是DNA降解导致将梅氏按蚊标本误鉴定为四带按蚊。首次检测时，PCR正确鉴定的总体概率为99.685%，与目前使用的其他基因方法相比具有优势。
