Silva Grasiene, Queiroga Felisbina L, Cruz Zélia, Maia Amana, Silvestre-Ferreira Ana C
Departamento de Ciências Veterinárias, Universidade de Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal.
Centro de Ciência Animal e Veterinária (CECAV), Universidade de Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal.
Animals (Basel). 2024 Jul 10;14(14):2031. doi: 10.3390/ani14142031.
Miranda's donkey, originating in northern Portugal, is an autochthonous breed that is deeply intertwined with the region's culture and economy. Knowledge of the physiological characteristics of the breed is important for its preservation, and several studies have been carried out, but none have focused on its coagulation profile. The aim of this study was to establish reference intervals (RIs) for coagulation in healthy Miranda's donkey and to assess the influence of sex and age. Blood samples from 75 clinically healthy animals were analyzed for seven coagulation parameters: four using IDEXX ProCyte Dx and three using Start 4-Diagnostica-Stago. The RI values were calculated following the ASVCP guidelines and with the Reference Advisor V.2.1 software. To analyze the influence of sex and age, SPSS version 29 was used. No significant differences were found between sexes ( > 0.05), but statistically significant differences were found between ages ( < 0.05) for platelet count and plateletcrit (both higher in young animals). The RIs described here can help monitor health and guide the diagnosis and treatment of diseased Miranda's donkeys, contributing to their preservation. Our study encourages further research on coagulation in donkeys and the use of different methodologies to obtain information for veterinarians working with this species.
米兰达驴原产于葡萄牙北部，是一个与该地区文化和经济紧密相连的本土品种。了解该品种的生理特征对其保护至关重要，已经开展了多项研究，但均未聚焦于其凝血指标。本研究的目的是建立健康米兰达驴凝血的参考区间（RIs），并评估性别和年龄的影响。对75只临床健康动物的血液样本进行了7项凝血参数分析：4项使用IDEXX ProCyte Dx检测，3项使用Start 4 - Diagnostica - Stago检测。参考区间值按照ASVCP指南并使用Reference Advisor V.2.1软件进行计算。为分析性别和年龄的影响，使用了SPSS 29版软件。性别之间未发现显著差异（> 0.05），但在年龄之间，血小板计数和血小板压积存在统计学显著差异（< 0.05）（年轻动物的这两项指标均较高）。这里描述的参考区间有助于监测健康状况，并指导患病米兰达驴的诊断和治疗，对其保护具有重要意义。我们的研究鼓励进一步开展关于驴凝血的研究，并采用不同方法为从事该物种工作的兽医获取信息。
