Xu Xinlei, Fan Suyu, Wu Hao, Li Haoyu, Shan Xiaoyu, Wang Mingfeng, Zhang Yang, Xu Qi, Chen Guohong
Key Laboratory for Evaluation and Utilization of Poultry Genetic Resources of Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
Animals (Basel). 2024 Jul 12;14(14):2042. doi: 10.3390/ani14142042.
China is a major goose-raising country, and the geese industry plays a significant role in animal husbandry. Therefore, goose growth performance (body weight) is a critical topic. Goose gut microbiota influences weight gain by regulating its energy metabolism and digestion. Additionally, the impact of cecal microbial community structure on goose growth and development, energy metabolism, and immunity has been examined. However, most studies have used different additives or feeds as variables. Improving the understanding of the dynamic changes in gut microbial communities in geese of different body weights during their growth and development and their correlation with the host's body weight is necessary. In this study, the cecal microbiota of healthy Yangzhou geese with large (L) and small (S) body weights, all at the same age (70 days old) and under the same feeding conditions, were sequenced using 16S rRNA. The sequencing results were annotated using QIIME2 (classify-sklearn algorithm) software, and the linkET package was used to explore the correlation between intestinal microorganisms and the body weight of the Yangzhou goose (Spearman). At the level, the / ratio in the large body weight group was approximately 20% higher than that in the small body weight group, with and exhibiting a highly significant negative correlation. At the level, constituted the most abundant microbial group in both groups, although the exhibited a higher abundance in the large than the small weight group. Spearman correlation analysis and the linkET package were used to analyze the correlation between cecal microflora and production performance indicators that showed significant differences between the two groups and showed that birth weight was significantly positively correlated with at the phylum level. At the genus level, leg and chest muscle weights exhibited significant positive correlations with , suggesting its critical role in promoting the growth and development of goose leg and chest muscles. A significant negative correlation was observed between and . These findings offer a crucial theoretical foundation for the study of gastrointestinal microorganisms and provide insights into the development and formulation of poultry probiotics.
中国是养鹅大国，鹅产业在畜牧业中发挥着重要作用。因此，鹅的生长性能（体重）是一个关键课题。鹅肠道微生物群通过调节其能量代谢和消化来影响体重增加。此外，盲肠微生物群落结构对鹅生长发育、能量代谢和免疫力的影响也已得到研究。然而，大多数研究将不同的添加剂或饲料作为变量。有必要进一步了解不同体重鹅在生长发育过程中肠道微生物群落的动态变化及其与宿主体重的相关性。在本研究中，对同一日龄（70日龄）且饲养条件相同的健康扬州大体重（L）和小体重（S）鹅的盲肠微生物群进行16S rRNA测序。测序结果使用QIIME2（classify-sklearn算法）软件进行注释，并使用linkET软件包探讨肠道微生物与扬州鹅体重之间的相关性（Spearman法）。在门水平上，大体重组的/比值比小体重组高约20%，且与呈现极显著负相关。在纲水平上，两组中均为最丰富的微生物类群，尽管在大体重组中的丰度高于小体重组。使用Spearman相关性分析和linkET软件包分析两组间存在显著差异的盲肠微生物群与生产性能指标之间的相关性，结果表明出生体重在门水平上与呈显著正相关。在属水平上，腿肌和胸肌重量与呈显著正相关，表明其在促进鹅腿肌和胸肌生长发育中的关键作用。与之间存在显著负相关。这些发现为胃肠道微生物的研究提供了重要的理论基础，并为家禽益生菌的开发和配方设计提供了见解。
