Wojtkowska Agata, Małek Anna, Giziński Sławomir, Sapierzyński Rafał, Rodo Anna, Sokołowska Justyna, Zabielska-Koczywąs Katarzyna A, Wojtalewicz Anna, Walewska Magdalena, Kautz Ewa, Ostrzeszewicz Magdalena, Lechowski Roman
Department of Small Animal Diseases with Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
Department of Large Animal Diseases with Clinic, Institute of Veterinary Medicine, Warsaw University of Life Science, 02-787 Warsaw, Poland.
Animals (Basel). 2024 Jul 19;14(14):2110. doi: 10.3390/ani14142110.
Feline injection-site sarcomas (FISSs) are aggressive neoplasms that have been associated mostly with vaccination. Feline noninjection-site sarcomas (non-FISSs) are less frequently observed in cats and may arise in any anatomic site. This study aimed to determine the differences in the expression of the selected proteins (matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), cyclooxygenase-2 (COX-2), and P-glycoprotein (PGP)) and their correlation with the mitotic count in FISS and non-FISS, in order to characterize their immunohistochemical features. A preliminary study of eleven samples of FISS and eight samples of non-FISS was performed using immunohistochemistry. Among all the tested sarcomas, 80.4% of the tumors were positive for COX-2, 90.2% were positive for MMP-9, and 100% were positive for PGP. The results showed that the expressions of COX-2, MMP-9, and PGP were significantly higher in FISS than in non-FISS (COX-2- ≤ 0.001; MMP-9- ≤ 0.05; and PGP- ≤ 0.05). A Spearman rank correlation analysis showed a moderate negative correlation between the expression of COX-2 and MMP-9 in FISS (r = -0.52). A strong negative correlation between COX-2 and PGP (r = -0.81), a moderate positive correlation between MMP-2 and MMP-9 (r = +0.69), and a moderate negative correlation between MMP-2 and PGP (r = -0.44) were observed in non-FISS. In summary, our study presents the immunohistochemical profile of the proteins involved with inflammation and carcinogenesis in FISS and non-FISS, which can contribute to expanding the knowledge of tumor biology.
猫注射部位肉瘤（FISSs）是侵袭性肿瘤，大多与疫苗接种有关。猫非注射部位肉瘤（非FISSs）在猫中较少见，可发生于任何解剖部位。本研究旨在确定所选蛋白质（基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9）、环氧合酶-2（COX-2）和P-糖蛋白（PGP））表达的差异及其与FISS和非FISS有丝分裂计数的相关性，以表征其免疫组织化学特征。使用免疫组织化学对11个FISS样本和8个非FISS样本进行了初步研究。在所有测试的肉瘤中，80.4%的肿瘤COX-2呈阳性，90.2%的肿瘤MMP-9呈阳性，100%的肿瘤PGP呈阳性。结果显示，FISS中COX-2、MMP-9和PGP的表达显著高于非FISS（COX-2 -≤0.001；MMP-9 -≤0.05；PGP -≤0.05）。Spearman等级相关分析显示，FISS中COX-2和MMP-9的表达呈中度负相关（r = -0.52）。在非FISS中观察到COX-2和PGP之间呈强负相关（r = -0.81），MMP-2和MMP-9之间呈中度正相关（r = +0.69），MMP-2和PGP之间呈中度负相关（r = -0.44）。总之，我们的研究展示了FISS和非FISS中与炎症和致癌作用相关蛋白质的免疫组织化学特征，这有助于扩展肿瘤生物学知识。
