Martins Joana P, da Silva Elisabete T, Fernandes António A, Costa de Oliveira Sofia
Associated Laboratory for Energy, Transports and Aerospace (LAETA), Institute of Science and Innovation in Mechanical and Industrial Engineering (INEGI), 4200-465 Porto, Portugal.
Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal.
Bioengineering (Basel). 2024 Jun 21;11(7):636. doi: 10.3390/bioengineering11070636.
Medical device-related infections (DRIs), especially prevalent among critically ill patients, impose significant health and economic burdens and are mainly caused by bacteria. Severe infections often necessitate device removal when antibiotic therapy is inefficient, delaying recovery. To tackle this issue, PCL drug-eluting coated meshes were explored, and they were printed via melt electrowriting (MEW). These meshes were coated with gentamicin sulfate (GS) and tetracycline hydrochloride (TCH) and underwent FTIR analysis to confirm drug integration. Antimicrobial activity was assessed via agar diffusion assays and biofilm formation assays against bacterial strains: ATCC 27853, ATCC 25922, ATCC 43300, and ATCC 35984. FTIR analysis evidenced the presence of the drugs in the meshes. TCH displayed broad-spectrum antimicrobial activity against all strains, whereas GS was effective against all except . These findings indicate the potential of cost-effective ultra-fine drug coating fibers for medical device applications, offering infection prevention during implantation. This preliminary study demonstrates the feasibility of producing drug-eluting fibers for DRI prevention through a non-toxic, fast, and cost-efficient technique, paving the way for enhanced patient care and reduced healthcare costs.
与医疗设备相关的感染（DRIs）在重症患者中尤为普遍，带来了巨大的健康和经济负担，且主要由细菌引起。当抗生素治疗无效时，严重感染往往需要移除设备，从而延迟康复。为了解决这个问题，人们探索了聚己内酯药物洗脱涂层网片，并通过熔体静电纺丝（MEW）进行打印。这些网片涂有硫酸庆大霉素（GS）和盐酸四环素（TCH），并进行了傅里叶变换红外光谱（FTIR）分析以确认药物整合。通过琼脂扩散试验和生物膜形成试验评估了对细菌菌株ATCC 27853、ATCC 25922、ATCC 43300和ATCC 35984的抗菌活性。FTIR分析证明了网片中存在药物。TCH对所有菌株均显示出广谱抗菌活性，而GS对除……外的所有菌株均有效。这些发现表明具有成本效益的超细药物涂层纤维在医疗设备应用中的潜力，可在植入过程中预防感染。这项初步研究证明了通过一种无毒、快速且经济高效的技术生产用于预防DRIs的药物洗脱纤维的可行性，为改善患者护理和降低医疗成本铺平了道路。
