Fenton Navarro Bertha, Casimiro Aguayo Alexis Abraham, Torres Gómez Yayr Luis, Cervantes Alfaro Miguel, Torner Luz
Laboratorio de Glicobiología y Farmacognosia, Facultad de Ciencias Médicas y Biológicas "Dr. Ignacio Chávez", Universidad Michoacana de San Nicolás de Hidalgo. Av. Dr. Rafael Carrillo S/N, Esq. Dr. Salvador González Herrejón Bosque, Cuauhtémoc, Morelia 58020, Michoacán, Mexico.
Laboratorio de Neurociencias, Facultad de Ciencias Médicas y Biológicas "Dr. Ignacio Chávez", Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58000, Michoacán, Mexico.
Antioxidants (Basel). 2024 Jul 2;13(7):802. doi: 10.3390/antiox13070802.
Early life stress (ELS) is a risk factor for the development of chronic diseases resulting from functional alterations of organs in the cardiorespiratory and renal systems. This work studied the changes in oxidative stress enzyme activities (EAs) of SOD, CAT, GPX, GR, GST, NOS, MDA, and FRAP in different organs (heart, liver, kidney, adrenal glands (AGs), and pancreas) of male and female Sprague-Dawley rat pups on postnatal day (PN) 15, immediately after basal and acute or chronic stress conditions were accomplished, as follows: basal control (BC; undisturbed maternal pups care), stress control (SC; 3 h maternal separation on PN15), basal maternal separation (BMS; daily 3 h maternal separation on PN 1-14), and stress maternal separation (SMS; daily 3 h maternal separation on PN 1-14 and 3 h maternal separation on PN15). Acute or long-term stress resulted in overall oxidative stress, increase in EA, and reduced antioxidant capacity in these organs. Some different response patterns, due to precedent SMS, were observed in specific organs, especially in the AGs. Acute stress exposure increases the EA, but chronic stress generates a response in the antioxidant system in some of the organs studied and is damped in response to a further challenge.
早期生活应激（ELS）是由心肺和肾脏系统器官功能改变导致的慢性疾病发生的一个风险因素。这项研究调查了出生后第15天（PN15）的雄性和雌性斯普拉格-道利大鼠幼崽在基础、急性或慢性应激条件完成后，不同器官（心脏、肝脏、肾脏、肾上腺（AGs）和胰腺）中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPX）、谷胱甘肽还原酶（GR）、谷胱甘肽S-转移酶（GST）、一氧化氮合酶（NOS）、丙二醛（MDA）和铁还原抗氧化能力（FRAP）的氧化应激酶活性（EAs）变化，具体如下：基础对照（BC；母鼠正常照料幼崽）、应激对照（SC；PN15时母鼠与幼崽分离3小时）、基础母鼠分离（BMS；PN1-14每天母鼠与幼崽分离3小时）和应激母鼠分离（SMS；PN1-14每天母鼠与幼崽分离3小时以及PN15时母鼠与幼崽分离3小时）。急性或长期应激导致这些器官出现整体氧化应激、酶活性增加以及抗氧化能力降低。由于先前的应激母鼠分离，在特定器官，尤其是肾上腺中观察到了一些不同的反应模式。急性应激暴露会增加酶活性，但慢性应激会在一些研究的器官中引发抗氧化系统的反应，并且在应对进一步的刺激时反应减弱。
