Rong Yongrong, Li Bing, Hou Yiran, Zhang Liqiang, Jia Rui, Zhu Jian
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
Antioxidants (Basel). 2024 Jul 15;13(7):849. doi: 10.3390/antiox13070849.
is a significant freshwater species with substantial nutritional and economic value. Rice-carp co-culture represents one of its principal cultivation methods. However, in the system, the optimal farming density for carp and the impact of high stocking density on their muscle nutritional composition have yet to be explored. Thus, the objective of the current study was to investigate the influences of stocking density on the muscle nutrient profiles and metabolism of in rice-fish co-culture systems. Common carp were cultured at three stocking densities, low density (LD), medium density (MD), and high density (HD), over a period of 60 days. Following this, comprehensive analyses incorporating physiological, biochemical, and multi-omics sequencing were conducted on the muscle tissue of . The results demonstrated that HD treatment led to a reduction in the antioxidant capacity of , while resulting in elevated levels of various fatty acids in muscle tissue, including saturated fatty acids (SFAs), omega-3 polyunsaturated fatty acids (n-3 PUFAs), and omega-6 polyunsaturated fatty acids (n-6 PUFAs). The metabolome analysis showed that HD treatment caused a marked reduction in 43 metabolites and a significant elevation in 30 metabolites, primarily linked to lipid and amino acid metabolism. Additionally, transcriptomic analysis revealed that the abnormalities in lipid metabolism induced by high-stocking-density treatment may be associated with significant alterations in the PPAR signaling pathway and adipokine signaling pathway. Overall, our findings indicate that in rice-fish co-culture systems, high stocking density disrupted the balance of antioxidant status and lipid metabolism in the muscles of
是一种具有重要营养和经济价值的淡水物种。稻鲤共作是其主要养殖方式之一。然而，在该系统中，鲤鱼的最佳养殖密度以及高放养密度对其肌肉营养成分的影响尚未得到探索。因此，本研究的目的是调查放养密度对稻鱼共作系统中鲤鱼肌肉营养成分和代谢的影响。将鲤鱼以低密度（LD）、中密度（MD）和高密度（HD）三种放养密度养殖60天。之后，对鲤鱼的肌肉组织进行了包括生理、生化和多组学测序在内的综合分析。结果表明，高密度处理导致鲤鱼抗氧化能力下降，同时肌肉组织中各种脂肪酸水平升高，包括饱和脂肪酸（SFA）、ω-3多不饱和脂肪酸（n-3 PUFA）和ω-6多不饱和脂肪酸（n-6 PUFA）。代谢组分析表明，高密度处理导致43种代谢物显著减少，30种代谢物显著增加，主要与脂质和氨基酸代谢有关。此外，转录组分析显示，高放养密度处理诱导的脂质代谢异常可能与PPAR信号通路和脂肪因子信号通路的显著改变有关。总体而言，我们的研究结果表明，在稻鱼共作系统中，高放养密度破坏了鲤鱼肌肉中抗氧化状态和脂质代谢的平衡。
