Tea Refining and Innovation Key Laboratory of Sichuan Province, Tea Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China.
Genes (Basel). 2024 Jul 17;15(7):932. doi: 10.3390/genes15070932.
Spider mite infestation has a severe impact on tea growth and quality. In this study, we conducted a deep exploration of the functions and regulations of the gene family using chromosomal localization and collinearity analysis. Additionally, we carefully examined the elements within these genes. To fully understand the metabolic response of under spider mite infection, we integrated previously published metabolomic and transcriptomic data. Our analysis revealed that multiple genes are associated with phospholipid metabolism, with showing the strongest correlation. Therefore, we employed qPCR and subcellular localization techniques to determine the expression pattern of this gene and its functional location within the cell. Overall, this study not only comprehensively elucidated the characteristics, structure, and evolution of the gene family but also identified several candidate genes related to phospholipid biosynthesis and associated with spider mites based on previously published data. This research makes a significant contribution to enhancing the resistance of tea to spider mite and maintaining optimal tea quality.
红蜘蛛虫害严重影响茶树的生长和品质。本研究利用染色体定位和共线性分析，深入探究了 基因家族的功能和规律，并对这些基因内的 元件进行了详细研究。为全面了解 受到红蜘蛛感染后的代谢反应，我们整合了先前发表的代谢组学和转录组学数据。分析表明，多个 基因与磷脂代谢相关，其中 相关性最强。因此，我们采用 qPCR 和亚细胞定位技术，确定了该基因的表达模式及其在细胞内的功能位置。综上所述，本研究不仅全面阐明了 基因家族的特征、结构和进化，还根据先前发表的数据，鉴定了几个与磷脂生物合成相关的候选 基因，这些基因与红蜘蛛有关。该研究为提高茶树对红蜘蛛的抗性和保持最佳茶叶品质做出了重要贡献。
