Genome-Wide Identification of the Gene Family in and Their Roles in Metabolic Regulation.

Spider mite infestation has a severe impact on tea growth and quality. In this study, we conducted a deep exploration of the functions and regulations of the gene family using chromosomal localization and collinearity analysis. Additionally, we carefully examined the elements within these genes. To fully understand the metabolic response of under spider mite infection, we integrated previously published metabolomic and transcriptomic data. Our analysis revealed that multiple genes are associated with phospholipid metabolism, with showing the strongest correlation. Therefore, we employed qPCR and subcellular localization techniques to determine the expression pattern of this gene and its functional location within the cell. Overall, this study not only comprehensively elucidated the characteristics, structure, and evolution of the gene family but also identified several candidate genes related to phospholipid biosynthesis and associated with spider mites based on previously published data. This research makes a significant contribution to enhancing the resistance of tea to spider mite and maintaining optimal tea quality.