Department of Internal Medicine, Pusan National University School of Medicine, Yangsan 50612, Republic of Korea.
Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan 50612, Republic of Korea.
Int J Mol Sci. 2024 Jul 12;25(14):7652. doi: 10.3390/ijms25147652.
Ischemia-reperfusion injury (IRI) in the kidneys is a major cause of acute kidney injury (AKI). Time-restricted feeding (TRF), known for its metabolic health benefits and alleviation of various chronic diseases without calorie restriction, was investigated for its potential protective effects against IRI-induced AKI. Male C57BL/6 mice underwent unilateral IRI, with their kidneys collected after two days. For two weeks before IRI induction, the TRF group had unlimited access to standard chow but within an 8-hour feeding window during the dark cycle. The study groups were Control, TRF, IRI, and TRF + IRI. In the TRF + IRI group, tubular damage scores significantly decreased compared to the IRI group. Furthermore, the TRF + IRI mice had lower levels of phosphorylated NF-κB and fewer F4/80-positive macrophages than the IRI group. Oxidative stress markers for lipids and proteins were also notably lower in the TRF + IRI group. Additionally, TUNEL-positive tubular cells and cleaved caspase-3 expression were reduced in the TRF + IRI group. Without calorie restriction, TRF mitigated renal damage by reducing inflammation, oxidative stress, and tubular apoptosis in renal IRI. This suggests that TRF could be a promising dietary strategy to prevent IRI-induced AKI.
肾脏的缺血再灌注损伤（IRI）是急性肾损伤（AKI）的主要原因。限时喂养（TRF）以其代谢健康益处和缓解各种慢性疾病而闻名，而无需限制热量，因此研究了其对IRI 诱导的 AKI 的潜在保护作用。雄性 C57BL/6 小鼠进行单侧 IRI，两天后采集肾脏。在 IRI 诱导前两周，TRF 组可无限制地摄入标准饲料，但在暗周期内的 8 小时进食窗口内进食。研究组为 Control、TRF、IRI 和 TRF + IRI。与 IRI 组相比，TRF + IRI 组的肾小管损伤评分显著降低。此外，TRF + IRI 组的磷酸化 NF-κB 和 F4/80 阳性巨噬细胞数量低于 IRI 组。TRF + IRI 组的脂质和蛋白质氧化应激标志物也明显降低。此外，TRF + IRI 组的 TUNEL 阳性肾小管细胞和 cleaved caspase-3 表达减少。无需限制热量，TRF 通过减少炎症、氧化应激和肾小管细胞凋亡来减轻肾脏 IRI 损伤。这表明 TRF 可能是预防 IRI 诱导的 AKI 的一种有前途的饮食策略。
