Zhang Jixin, Zhang Lan, Xu Huiqing, Wang Jun
College of Tourism and Culinary Institute, Yangzhou University, Yangzhou 225127, China.
Foods. 2024 Jul 11;13(14):2192. doi: 10.3390/foods13142192.
TGase-7S gels prepared after different HIU pretreatment times were used to intervene in healthy mice to analyze their effects on growth characteristics and intestinal morphology, and 16S rRNA high-throughput sequencing was applied to fecal samples to investigate the effects of the gel on the structure and diversity of intestinal flora in mice. The results showed that the intestinal tissues of mice in different treatment groups showed better integrity, and the intake of gel increased the length of small intestinal villi in mice, among which the 30-gel group had the highest value of villi length (599.27 ± 44.28) μm ( < 0.05) and showed the neatest and tightest arrangement, indicating that the intake of gel did not have adverse effects on the intestinal tract. The effect of gel ingestion on the diversity of the intestinal microbial community structure was more significant, positively promoting the growth of beneficial bacteria such as , , and . In addition, the ingestion of the gel improved the intestinal health of mice by altering the physiological functions of the intestinal flora and modulating their participation in various metabolic pathways. The above findings provide some theoretical value for the safety of 7S gel in food applications.
使用不同超声预处理时间后制备的转谷氨酰胺酶-7S凝胶干预健康小鼠，以分析其对生长特性和肠道形态的影响，并对粪便样本应用16S rRNA高通量测序，以研究该凝胶对小鼠肠道菌群结构和多样性的影响。结果显示，不同处理组小鼠的肠道组织显示出更好的完整性，凝胶的摄入增加了小鼠小肠绒毛的长度，其中30-凝胶组的绒毛长度值最高（599.27±44.28）μm（<0.05），且排列最整齐紧密，表明凝胶的摄入对肠道没有不良影响。凝胶摄入对肠道微生物群落结构多样性的影响更为显著，正向促进了如、和等有益菌的生长。此外，凝胶的摄入通过改变肠道菌群的生理功能并调节其参与各种代谢途径，改善了小鼠的肠道健康。上述发现为7S凝胶在食品应用中的安全性提供了一定的理论价值。
