Mizuno K, Ojima M, Hashimoto S, Watari H, Tani M, Satoh M, Fukuchi S
Life Sci. 1985 Dec 16;37(24):2297-304. doi: 10.1016/0024-3205(85)90021-9.
Immunoreactive renin was demonstrated in pituitary tissues of postmortem human subjects with different diseases. The specific immunoreactive renin activity comprised the majority of the tissue renin-like activity (mean, 83%), indicating the absence of nonspecific actions of proteases such as cathepsin D. We used three pituitary specimens with high levels of the specific renin activity for further biochemical characterization of the enzyme. Small differences were found in the molecular mass (45 K, 42 K and 37 K), binding to concanavalin A-Sepharose, and isoelectric points (pI) (4.72, 4.78, 4.86, 5.06, 5.28 and 5.44). These results seem to be interpreted as evidence for the presence of specific renin in the human pituitary with microheterogeneity.
在患有不同疾病的人类尸体的垂体组织中证实了免疫反应性肾素。特异性免疫反应性肾素活性占组织肾素样活性的大部分（平均83%），表明不存在诸如组织蛋白酶D等蛋白酶的非特异性作用。我们使用了三个具有高水平特异性肾素活性的垂体标本对该酶进行进一步的生化特性分析。在分子量（45K、42K和37K）、与伴刀豆球蛋白A-琼脂糖的结合以及等电点（pI）（4.72、4.78、4.86、5.06、5.28和5.44）方面发现了微小差异。这些结果似乎可解释为人类垂体中存在具有微异质性的特异性肾素的证据。
