Popa Laura Claudia, Farcas Simona Sorina, Andreescu Nicoleta Ioana
Department of Microscopic Morphology, Discipline of Genetics, Genomic Medicine Centre "Victor Babeș", University of Medicine and Pharmacy, 300041 Timisoara, Romania.
"Louis Turcanu" Children Emergency Hospital, 300011 Timisoara, Romania.
J Pers Med. 2024 Jul 3;14(7):717. doi: 10.3390/jpm14070717.
Cytochrome P450 1A2 (CYP1A2) is known to be the main enzyme directly responsible for caffeine metabolism. Rs762551 (NC_000015.10:g.74749576C>A) is a single nucleotide polymorphism of the gene, and it is known mainly for metabolizing caffeine. A significant worldwide health issue, type 2 diabetes (T2DM), has been reported to be negatively associated with coffee consumption. Yet, some studies have proven that high intakes of coffee can lead to a late onset of T2DM.
This study aims to find any significant correlations among polymorphism, coffee consumption, and T2DM.
A total of 358 people were enrolled in this study-218 diagnosed with T2DM, and 140 representing the control sample. The qPCR technique was performed, analyzing rs762551 (assay C_8881221) on the LightCycler 480 (Roche, Basel, Switzerland) with Gene Scanning software version 1.5.1 (Roche).
Our first observation was that the diabetic patients were likely to consume more coffee than the non-diabetic subjects. People with the AA genotype, or the fast metabolizers, are the least common, yet they are the highest coffee consumers and present the highest glucose and cholesterol levels. Another important finding is the correlation between coffee intake and glucose level, which showed statistically significant differences between the diabetic group ( = 0.0002) and the control group ( = 0.029).
The main conclusion of this study is that according to genotype, caffeine levels, glucose, and cholesterol are interconnected and proportionally related, regardless of type 2 diabetes.
细胞色素P450 1A2（CYP1A2）是已知直接负责咖啡因代谢的主要酶。Rs762551（NC_000015.10:g.74749576C>A）是该基因的单核苷酸多态性，主要以代谢咖啡因而闻名。2型糖尿病（T2DM）是一个重大的全球性健康问题，据报道与咖啡消费呈负相关。然而，一些研究证明，大量饮用咖啡会导致T2DM发病较晚。
本研究旨在发现该多态性、咖啡消费与T2DM之间的任何显著相关性。
本研究共纳入358人——218例被诊断为T2DM，140例作为对照样本。采用qPCR技术，使用基因扫描软件版本1.5.1（罗氏公司，瑞士巴塞尔）在LightCycler 480（罗氏公司，瑞士巴塞尔）上分析rs762551（检测C_8881221）。
我们的第一个观察结果是，糖尿病患者比非糖尿病受试者更有可能饮用更多咖啡。AA基因型的人，即快速代谢者，是最不常见的，但他们是咖啡消费量最高的人群，且血糖和胆固醇水平最高。另一个重要发现是咖啡摄入量与血糖水平之间的相关性，糖尿病组（P = 0.0002）和对照组（P = 0.029）之间存在统计学显著差异。
本研究的主要结论是，根据基因型，无论是否患有2型糖尿病，咖啡因水平、血糖和胆固醇都是相互关联且成比例相关的。
