Department of Applied Bioresource Science, The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Ehime 790-8566, Japan; Department of Agro-industrial Technology, Faculty of Agricultural Technology, Gadjah Mada University, Yogyakarta 55281, Indonesia.
Department of Bioscience, Graduate School of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan.
Mol Immunol. 2020 Feb;118:201-209. doi: 10.1016/j.molimm.2019.12.020. Epub 2019 Dec 30.
Trigonelline, one of the alkaloids contained in coffee, is important not only as one of the constituents of aroma and flavor in coffee but also as a useful source of nutrition. Its anti-microbial, anti-carcinogenic, and anti-hyperglycemic effects have been investigated in previous studies. However, there have not been any studies examining the anti-degranulation effect of trigonelline. In this study, the anti-degranulation effect of trigonelline was evaluated in in vitro and in vivo models using a rat basophilic leukemia cell line, RBL-2H3 cells, and a passive cutaneous anaphylaxis (PCA) reaction in mice, respectively. In the β-hexosaminidase release assay, trigonelline effectively suppressed antigen-induced degranulation of RBL-2H3 cells in a dose-dependent manner without cytotoxicity. Trigonelline also inhibited FcεRI-mediated intracellular signaling pathways, such as phosphorylation of PLCγ1, PI3 K, and Akt, in antigen-stimulated RBL-2H3 cells and suppressed the PCA response in mice. Moreover, trigonelline also inhibited the microtubule formation in RBL-2H3 cells, indicating that trigonelline could inhibit IgE-sensitized mast cell degranulation by attenuating both the intracellular calcium-dependent and independent pathways. These results revealed that trigonelline possesses the anti-degranulation effect against the development of allergic diseases.
咖啡中含有的生物碱之一——六氢吡啶，不仅是咖啡香气和风味的组成部分之一，也是一种有用的营养来源。之前的研究已经探讨了其抗菌、抗癌和降血糖作用。然而，目前还没有研究检查六氢吡啶的脱颗粒作用。在这项研究中，使用大鼠嗜碱性白血病细胞系 RBL-2H3 细胞和小鼠被动皮肤过敏反应 (PCA) 反应，分别在体外和体内模型中评估了六氢吡啶的脱颗粒作用。在β-己糖胺酶释放试验中，六氢吡啶以剂量依赖的方式有效抑制抗原诱导的 RBL-2H3 细胞脱颗粒，而没有细胞毒性。六氢吡啶还抑制了 FcεRI 介导的细胞内信号通路，如 PLCγ1、PI3K 和 Akt 的磷酸化，在抗原刺激的 RBL-2H3 细胞中，并抑制了小鼠的 PCA 反应。此外，六氢吡啶还抑制了 RBL-2H3 细胞中的微管形成，表明六氢吡啶可以通过减弱细胞内钙依赖性和非依赖性途径来抑制 IgE 敏化肥大细胞脱颗粒。这些结果表明，六氢吡啶具有抗脱颗粒作用，可以抑制过敏疾病的发展。
