Homayoun P, Roux F, Niel E, Bourre J M
Neurosci Lett. 1985 Nov 20;62(1):143-7. doi: 10.1016/0304-3940(85)90298-8.
Lipid biosynthesis was investigated in isolated cerebral microvessels obtained from adult Sprague-Dawley rats using [1-14C]acetate as precursor. All lipid classes were labelled by [1-14C]acetate. Neutral lipids incorporated about 50% of radiolabelled acetate, among which free fatty acids and triglycerids showed the highest level of incorporation. Moreover, about 4% of radioactivity was found in cholesterol fraction. In phospholipid fraction, phosphatidylcholine and phosphatidylethanolamine were the main radiolabelled phospholipids. [1-14C]acetate was also incorporated into sulphatides and cerebrosides. The presence of bovine serum albumin in incubation medium modified the percentage of incorporation in different lipid fractions.
利用[1-¹⁴C]乙酸盐作为前体，对从成年Sprague-Dawley大鼠分离得到的脑微血管中的脂质生物合成进行了研究。所有脂质类别都被[1-¹⁴C]乙酸盐标记。中性脂质掺入了约50%的放射性标记乙酸盐，其中游离脂肪酸和甘油三酯的掺入水平最高。此外，在胆固醇部分中发现了约4%的放射性。在磷脂部分中，磷脂酰胆碱和磷脂酰乙醇胺是主要的放射性标记磷脂。[1-¹⁴C]乙酸盐也掺入到硫脂和脑苷脂中。孵育培养基中牛血清白蛋白的存在改变了不同脂质部分的掺入百分比。
