大肠杆菌K12中的持液恢复。I - 缓冲液保存期间释放的物质。
Liquid holding recovery in E. coli K12. I - Substances released during buffer holding.
作者信息
Aragão B R, Gomes R A
出版信息
Rev Bras Pesqui Med Biol. 1979 Jun;12(2-3):117-22.
It is known that the incubation, in a buffer, of UV-irradiated E. coli cells results in viability increase, this phenomenon had been called liquid holding recovery (LHR). We have studied the cellular constituents release during LHR and verified that releasing rate is dose-dependent. LHR was also observed after nitrogen-mustard treatment and it is not blocked by caffeine. So, we suggest that LHR expression is not always a rec-gene dependent function and, probably, the survival increase could be explained by (a) DNA-repair, (b) reversible membrane damage and (c) cellular multiplication.
已知在缓冲液中对紫外线照射的大肠杆菌细胞进行孵育会导致活力增加，这种现象被称为液体保持恢复（LHR）。我们研究了LHR过程中细胞成分的释放，并证实释放速率是剂量依赖性的。在氮芥处理后也观察到了LHR，并且它不受咖啡因的阻断。因此，我们认为LHR的表达并不总是依赖于rec基因的功能，而且存活增加可能可以通过以下几点来解释：（a）DNA修复，（b）可逆性膜损伤，以及（c）细胞增殖。
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