Lee Yewon, Yoon Yohan, Choi Kyoung-Hee
Risk Analysis Research Center, Sookmyung Women's University, Seoul 04310, Republic of Korea.
Department of Food and Nutrition, Sookmyung Women's University, Seoul 04310, Republic of Korea.
Microorganisms. 2024 Jun 25;12(7):1290. doi: 10.3390/microorganisms12071290.
To decrease periodontal pathogens and increase the number of beneficial bacteria, probiotics and bioactive compounds made via microbial bioconversion are recently used. In addition, the interest regarding probiotics-mediated bioconversion with popular medicinal plants is increasing. , a type of wormwood, has recently been attention as a medicinal plant due to its various bioactive compounds. Therefore, we developed bioconverted milk containing that effectively inhibited periodontal pathogens and α-glucosidase. To select the appropriate lactic acid bacteria for the probiotic candidate strain, 74 strains of lactic acid bacteria were screened. Among them, SMFM2016-RK was chosen as the probiotic due to its beneficial characteristics such as high acid and bile tolerance, antioxidant activity, and α-glucosidase inhibition. Based on the minimal bactericidal concentration against three periodontal pathogens, the following appropriate concentrations of extract were added to milk: 5 mg/mL of ethanol extract and 25 mg/mL of hot-water extract. Four bioconverted milks (BM), BM1, BM2, BM3, and BM4, were produced by combining SMFM2016-RK alone, SMFM2016-RK and ethanol extract, SMFM2016-RK and hot-water extract, and SMFM2016-RK with both extracts. As a result of antimicrobial activity, BM3 inhibited the growth of and the most, and BM4 suppressed the growth of the most. In addition, bioconverted milk containing (BM2, BM3, and BM4) inhibited α-glucosidase more effectively than BM1. The whole genome of SMFM2016-RK was obtained, and 3135 CDS, 67 tRNA, and 16 RNA were predicted. The genome annotation of SMFM2016-RK revealed 11 CDS related to proteolysis and amino acid metabolism and 2 CDS of phenolic acid-metabolizing enzymes. In conclusion, -added milk bioconverted by SMFM2016-RK displayed both the growth inhibitory effect on periodontal pathogens and the α-glucosidase inhibitory activity; thus, it necessitates to evaluate the effects on the alleviation of periodontal diseases and glycemic control through future animal experiments.
为了减少牙周病原体并增加有益细菌的数量，最近开始使用通过微生物生物转化制备的益生菌和生物活性化合物。此外，人们对益生菌介导的与常见药用植物的生物转化的兴趣也在增加。艾草作为一种药用植物，由于其多种生物活性化合物，最近受到了关注。因此，我们开发了含有艾草的生物转化牛奶，其能有效抑制牙周病原体和α-葡萄糖苷酶。为了选择合适的乳酸菌作为益生菌候选菌株，筛选了74株乳酸菌。其中，SMFM2016-RK因其高酸和胆汁耐受性、抗氧化活性以及α-葡萄糖苷酶抑制等有益特性而被选为益生菌。基于对三种牙周病原体的最低杀菌浓度，向牛奶中添加了以下合适浓度的艾草提取物：5mg/mL的艾草乙醇提取物和25mg/mL的艾草热水提取物。通过单独组合SMFM2016-RK、SMFM2016-RK和乙醇提取物、SMFM2016-RK和热水提取物以及SMFM2016-RK与两种提取物，制备了四种生物转化牛奶（BM），即BM1、BM2、BM3和BM4。抗菌活性结果表明，BM3对牙龈卟啉单胞菌和具核梭杆菌的生长抑制作用最强，BM4对伴放线聚集杆菌的生长抑制作用最强。此外，含有艾草的生物转化牛奶（BM2、BM3和BM4）比BM1更有效地抑制α-葡萄糖苷酶。获得了SMFM2016-RK的全基因组，预测有3135个编码序列（CDS）、67个转运RNA（tRNA）和16个核糖体RNA（rRNA）。SMFM2016-RK的基因组注释显示有11个与蛋白水解和氨基酸代谢相关的CDS以及2个酚酸代谢酶的CDS。总之，由SMFM2016-RK生物转化的添加艾草的牛奶对牙周病原体具有生长抑制作用，同时具有α-葡萄糖苷酶抑制活性；因此，有必要通过未来的动物实验评估其对缓解牙周疾病和血糖控制的影响。
