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猪精液外泌体的蛋白质组学和代谢特征揭示了与精子活力相关的生物标志物候选物。

Proteomics and Metabolic Characteristics of Boar Seminal Plasma Extracellular Vesicles Reveal Biomarker Candidates Related to Sperm Motility.

机构信息

State Key Laboratory of Animal Biotech Breeding, College of Animal Science & Technology, China Agricultural University, Beijing 100193, P. R. China.

出版信息

J Proteome Res. 2024 Sep 6;23(9):3764-3779. doi: 10.1021/acs.jproteome.4c00060. Epub 2024 Jul 27.

DOI:10.1021/acs.jproteome.4c00060
PMID:39067049
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11385425/
Abstract

Although seminal plasma extracellular vesicles (SPEVs) play important roles in sperm function, little is known about their metabolite compositions and roles in sperm motility. Here, we performed metabolomics and proteomics analysis of boar SPEVs with high or low sperm motility to investigate specific biomarkers affecting sperm motility. In total, 140 proteins and 32 metabolites were obtained through differentially expressed analysis and weighted gene coexpression network analysis (WGCNA). Seven differentially expressed proteins (DEPs) (ADIRF, EPS8L1, PRCP, CD81, PTPRD, CSK, LOC100736569) and six differentially expressed metabolites (DEMs) (adenosine, beclomethasone, 1,2-benzenedicarboxylic acid, urea, 1-methyl-l-histidine, and palmitic acid) were also identified in WGCNA significant modules. Joint pathway analysis revealed that three DEPs (GART, ADCY7, and NTPCR) and two DEMs (urea and adenosine) were involved in purine metabolism. Our results suggested that there was significant correlation between proteins and metabolites, such as IL4I1 and urea ( = 0.86). Furthermore, we detected the expression level of GART, ADCY7, and CDC42 in sperm of two groups, which further verified the experimental results. This study revealed that several proteins and metabolites in SPEVs play important roles in sperm motility. Our results offered new insights into the complex mechanism of sperm motility and identified potential biomarkers for male reproductive diseases.

摘要

虽然精浆细胞外囊泡 (SPEV) 在精子功能中发挥着重要作用，但对于其代谢产物组成及其在精子运动中的作用知之甚少。在这里，我们对具有高或低精子运动能力的公猪 SPEV 进行了代谢组学和蛋白质组学分析，以研究影响精子运动能力的特定生物标志物。通过差异表达分析和加权基因共表达网络分析（WGCNA），共获得 140 种蛋白质和 32 种代谢物。7 种差异表达蛋白（ADIRF、EPS8L1、PRCP、CD81、PTPRD、CSK、LOC100736569）和 6 种差异表达代谢物（腺苷、倍氯米松、1,2-苯二甲酸、尿素、1-甲基-L-组氨酸和棕榈酸）也在 WGCNA 显著模块中得到鉴定。联合途径分析表明，3 种差异表达蛋白（GART、ADCY7 和 NTPCR）和 2 种差异表达代谢物（尿素和腺苷）参与嘌呤代谢。我们的结果表明，蛋白质和代谢物之间存在显著相关性，例如 IL4I1 和尿素（=0.86）。此外，我们检测了两组精子中 GART、ADCY7 和 CDC42 的表达水平，这进一步验证了实验结果。这项研究表明，SPEV 中的几种蛋白质和代谢物在精子运动中起着重要作用。我们的结果为精子运动的复杂机制提供了新的见解，并确定了男性生殖疾病的潜在生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdbb/11385425/9282aefcfd70/pr4c00060_0008.jpg
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3
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