Molecular, Cellular, and Developmental Biology Department, University of California, Santa Barbara, Santa Barbara, CA 93106, USA.
Kavli Institute for Theoretical Physics and Department of Physics, University of California, Santa Barbara, Santa Barbara, CA 93106, USA.
STAR Protoc. 2024 Sep 20;5(3):103048. doi: 10.1016/j.xpro.2024.103048. Epub 2024 Jul 27.
Drosophila border cell clusters model collective cell migration. Airyscan super-resolution microscopy enables fine-scale description of cluster shape and texture. Here we describe how to convert Airyscan images of border cell clusters into 3D models of the surface and detect regions of convex and concave curvature. We use spectral decomposition analysis to compare surface textures across genotypes to determine how genes of interest impact cluster surface geometry. This protocol applies to border cells and could generalize to additional cell types. For complete details on the use and execution of this protocol, please refer to Gabbert et al..
果蝇缘细胞簇模型集体细胞迁移。Airyscan 超分辨率显微镜能够对细胞簇的形状和纹理进行精细描述。本文介绍了如何将缘细胞簇的 Airyscan 图像转换为表面的 3D 模型，并检测凸面和凹面曲率的区域。我们使用谱分解分析来比较不同基因型的表面纹理，以确定感兴趣的基因如何影响细胞簇表面几何形状。该方案适用于缘细胞，也可推广到其他细胞类型。有关该方案使用和执行的完整详细信息，请参阅 Gabbert 等人的文章。
