鞘内途径递呈蛋白、肽和 shRNA 在实验性变应性脑脊髓炎小鼠模型中的应用方案。
Protocol for delivering proteins, peptides, and shRNAs via the intrathecal route in the experimental allergic encephalomyelitis mouse model.
机构信息
Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea; BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea; Brain Science and Engineering Institute, Kyungpook National University, Daegu 41944, Republic of Korea.
Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea; BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea; Brain Science and Engineering Institute, Kyungpook National University, Daegu 41944, Republic of Korea.
出版信息
STAR Protoc. 2024 Sep 20;5(3):103219. doi: 10.1016/j.xpro.2024.103219. Epub 2024 Jul 27.
The blood-brain barrier hinders drug delivery to the central nervous system (CNS), particularly for large molecules. Here, we present a protocol for delivering proteins, peptides, and short hairpin RNAs (shRNAs) via the intrathecal (IT) route in the experimental allergic encephalomyelitis (EAE) mouse model. We describe steps for developing EAE in mice and administering treatments intrathecally. The insights into treatment efficacy that can be provided by this protocol make it an important tool for CNS research. For complete details on the use and execution of this protocol, please refer to Bhusal et al..
血脑屏障阻碍了药物向中枢神经系统（CNS）的输送，尤其是对于大分子药物而言。在此，我们提供了一种通过鞘内（IT）途径在实验性变态反应性脑脊髓炎（EAE）小鼠模型中递送蛋白质、肽和短发夹 RNA（shRNA）的方案。我们描述了在小鼠中建立 EAE 模型和鞘内给予治疗的步骤。通过该方案可以深入了解治疗效果，这使其成为 CNS 研究的重要工具。如需详细了解该方案的使用和执行，请参考 Bhusal 等人的研究。
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