College of Life Science, Northeast Agricultural University, Harbin 150030, China.
College of Life Science, Northeast Agricultural University, Harbin 150030, China.
Int J Biol Macromol. 2024 Oct;277(Pt 2):134192. doi: 10.1016/j.ijbiomac.2024.134192. Epub 2024 Jul 26.
The intricate structure of lignin in straw makes it challenging to hydrolyze, making it a key focus of current research. However, there has been limited study on the effect of enzyme inducer (MnSO) combined with functional microorganisms on lignin degradation during straw composting. Based on this, four composting treatment groups were set up in this study. Control (CK), functional microorganism addition treatment (F), Mn enzyme inducer (Mn), and Mn enzyme inducer coupled with functional microorganism addition treatment (FMn) were tested for composting. Manganese(II)-coupled microorganisms improved lignin degradation: FMn > Mn > F > CK. They increased the lignin loss rate from 25.54 % to 42.61 %. Laccase activity increased from 3.45 to 43.74 U/g and manganese peroxidase activity increased from 145.52 to 264.91 U/g. And gene abundance was increased. Microbial community structure and dominant genera changed. Structural equations support the idea that functional microorganisms coupled with manganese can modify physicochemical indices, thereby regulating gene expression and enhancing enzyme activity. Furthermore, the stimulation of fungal growth and increased extracellular laccase and manganese peroxidase activities can affect the degradation of lignin. This study provides new insights and theoretical support for efficient lignin degradation and efficient resource utilization of compost products.
秸秆木质素结构复杂，水解困难，是当前研究的重点。然而，关于酶诱导剂（MnSO）与功能微生物结合对秸秆堆肥过程中木质素降解的影响研究较少。基于此，本研究设置了 4 个堆肥处理组，分别为对照（CK）、添加功能微生物（F）、添加 Mn 酶诱导剂（Mn）和 Mn 酶诱导剂与功能微生物联合添加（FMn），进行堆肥试验。Mn(II)结合微生物可提高木质素降解效果：FMn＞Mn＞F＞CK。木质素损失率从 25.54%增加到 42.61%。漆酶活性从 3.45 U/g 增加到 43.74 U/g，锰过氧化物酶活性从 145.52 U/g 增加到 264.91 U/g。同时，基因丰度增加。微生物群落结构和优势属发生变化。结构方程表明，功能微生物与锰结合可以改变理化指标，从而调节基因表达，增强酶活性。此外，真菌生长的刺激和胞外漆酶和锰过氧化物酶活性的增加会影响木质素的降解。本研究为高效木质素降解和堆肥产品的高效资源利用提供了新的见解和理论支持。
