College of Life Science, Jilin Agricultural University, Changchun 130118, China; Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China; Key Laboratory of Straw Comprehensive Utilization and Black Soil Conservation, Ministry of Education, Changchun 130012, China.
College of Life Science, Jilin Agricultural University, Changchun 130118, China.
Bioresour Technol. 2022 Oct;361:127699. doi: 10.1016/j.biortech.2022.127699. Epub 2022 Jul 26.
Lignin of high purity and structural integrity was isolated from the enzymatic residue of corn stover. Degradation of the lignin by laccase, lignin peroxidase, and manganese peroxidase was investigated. Structural changes in the lignin after degradation were characterized by scanning electron microscopy, nitrogen adsorption and Fourier transform infrared spectroscopy, and the enzymatic products were systematically analyzed by gas chromatography mass spectrometry. The highest percentage of lignin degradation was obtained with a mixture of three enzymes (25.79%): laccase (Lac), the starting enzyme of the mixed enzyme reaction, worked with lignin peroxidase (LiP), and manganese peroxidase (MnP) to further degrade lignin. This degradation destroyed the macromolecular structure of lignin, broke its key chemical bonds, and opened benzene rings, thus producing more acidic compounds. This study elucidated the concept of degrading lignin from corn stover using the Lac, LiP and MnP enzymes synergistically, thus providing a theoretical basis for the biodegradation of lignin.
从玉米秸秆的酶解残渣中分离出高纯度和结构完整的木质素。研究了漆酶、木质素过氧化物酶和锰过氧化物酶对木质素的降解作用。通过扫描电子显微镜、氮吸附和傅里叶变换红外光谱对木质素降解后的结构变化进行了表征，并通过气相色谱-质谱对酶解产物进行了系统分析。在混合三种酶（25.79%）的情况下，木质素的降解率最高：漆酶（Lac）是混合酶反应的起始酶，与木质素过氧化物酶（LiP）和锰过氧化物酶（MnP）一起进一步降解木质素。这种降解破坏了木质素的大分子结构，打破了其关键化学键，并打开了苯环，从而产生了更多的酸性化合物。本研究阐明了利用漆酶、木质素过氧化物酶和锰过氧化物酶协同降解玉米秸秆木质素的概念，为木质素的生物降解提供了理论依据。
