Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Hohai University, Nanjing 210098, PR China; College of Environment, Hohai University, Nanjing 210098, PR China.
Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Hohai University, Nanjing 210098, PR China; College of Environment, Hohai University, Nanjing 210098, PR China.
Bioresour Technol. 2024 Sep;408:131140. doi: 10.1016/j.biortech.2024.131140. Epub 2024 Jul 26.
The long acclimation period and sensitivity to environmental conditions of Anammox are the bottlenecks for its promotion and application. An innovative strategy was adopted to accelerate functional microbial enhancement and improve nitrogen removal performance by inoculating cryopreserved Anammox sludge and activated sludge with intermittent dosing of nanoscale zero-valent iron (nZVI). The acclimation time was shortened by 76 days with nitrogen removal efficiency (NRE) reaching up to 91.07 %. Anammox, NDFO (nitrate/nitrite-dependent Fe(II) oxidation), Feammox (Fe(III) reduction coupled with anaerobic ammonium oxidation) and abiotic reactions were coupled in the system with nZVI, contributing to 69.79 %, 15.14 %, 9.84 % and 0.25 % of nitrogen removal, respectively. Further microbial analysis demonstrated significant enrichment of functional microorganisms, such as Candidatus Jettenia, Acidovorax and Comamonas. High-efficient nitrogen removal was attribute to the increase of functional genes involved in Anammox, electronic transfer, heme C synthesis and iron metabolism. This work provides an inspiring idea for the mainstream Anammox application.
厌氧氨氧化菌的长期驯化期和对环境条件的敏感性是其推广和应用的瓶颈。本研究采用一种创新策略，通过间歇投加纳米零价铁（nZVI）来接种冷冻厌氧氨氧化污泥和活性污泥，以加速功能微生物的增强并提高氮去除性能。驯化时间缩短了 76 天，氮去除效率（NRE）最高可达 91.07%。在投加 nZVI 的系统中，厌氧氨氧化、硝酸盐/亚硝酸盐依赖型 Fe(II)氧化（NDFO）、Feammox（Fe(III)还原耦合厌氧氨氧化）和非生物反应耦合在一起，分别贡献了 69.79%、15.14%、9.84%和 0.25%的氮去除。进一步的微生物分析表明，功能微生物如杰顿氏菌属（Candidatus Jettenia）、食酸菌属（Acidovorax）和丛毛单胞菌属（Comamonas）得到了显著富集。高效的氮去除归因于参与厌氧氨氧化、电子传递、血红素 C 合成和铁代谢的功能基因的增加。这项工作为主流厌氧氨氧化的应用提供了一个鼓舞人心的思路。
