National Institute of Public Health of Mexico, Av. Universidad 655, Morelos, Cuernavaca 62130, Mexico.
National Institute of Public Health of Mexico, Av. Universidad 655, Morelos, Cuernavaca 62130, Mexico.
Environ Toxicol Pharmacol. 2024 Sep;110:104521. doi: 10.1016/j.etap.2024.104521. Epub 2024 Jul 26.
The ability of organophosphate pesticides to disturb immune function has been demonstrated by in vivo and in vitro studies, but evidence of such effects on humans remains scarce. To assess the association between organophosphate pesticides exposure and cytokine levels in Mexican flower workers, a cross-sectional study was carried out. A questionnaire was provided to 121 male flower workers, and urine and blood samples were collected. Using gas chromatography, urinary concentrations of dialkylphosphate metabolites were determined. The serum cytokine levels, IL-4, IL-5, IL-6, IL-8, and IL-10, were measured using multiplex analysis, and levels of INF-γ and TNF-α by ELISA. We found that a higher dialkylphosphate concentration decreased the pro-inflammatory cytokines INF-γ (β = -0.63; 95 % CI: -1.22, -0.05), TNF-α (β= -1.18; 95 % CI: -2.38, 0.02), and IL-6 (β= -0.59; 95 % CI: -1.29, 0.12), and increased IL-10 (β=0.56; 95 % CI: 0.02, 1.09), the main anti-inflammatory cytokine, suggesting an imbalance of the immune response in flower workers.
有机磷农药具有干扰免疫功能的能力，这已在体内和体外研究中得到证实，但关于此类作用对人类的影响的证据仍然很少。为了评估墨西哥花卉工人接触有机磷农药与细胞因子水平之间的关联，进行了一项横断面研究。向 121 名男性花卉工人提供了一份问卷，并采集了尿液和血液样本。使用气相色谱法测定尿中二烷基磷酸盐代谢物的浓度。使用多重分析测定血清细胞因子水平，IL-4、IL-5、IL-6、IL-8 和 IL-10，使用 ELISA 测定 INF-γ 和 TNF-α的水平。我们发现，较高的二烷基磷酸盐浓度降低了促炎细胞因子 INF-γ（β= -0.63；95%CI：-1.22，-0.05）、TNF-α（β= -1.18；95%CI：-2.38，0.02）和 IL-6（β= -0.59；95%CI：-1.29，0.12），并增加了主要抗炎细胞因子 IL-10（β=0.56；95%CI：0.02，1.09），这表明花卉工人的免疫反应失衡。
