He Jing, Wang Ting, Liu Danyang, Yang Jun, He Yuanpei, Zhao Shouliang, Ju Yanqin
Department of Stomatology, Huashan Hospital, Fudan University, Shanghai, China.
Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
Int J Stem Cells. 2024 Nov 30;17(4):437-448. doi: 10.15283/ijsc23205. Epub 2024 Jul 29.
Schwann cells (SCs), a type of glial cell in the peripheral nervous system, can serve as a source of mesenchymal stem cells (MSCs) to repair injured pulp. This study aimed to investigate the role of SCs in tooth germ development and repair of pulp injury. We performed RNA-seq and immunofluorescent staining on tooth germs at different developmental stages. The effect of L-type calcium channel (LTCC) blocker nimodipine on SCs odontogenic differentiation was analyzed by real-time polymerase chain reaction and Alizarin Red S staining. We used the PLP1-CreERT2/ Rosa26-GFP tracing mice model to examine the role of SCs and Ca1.2 in self-repair after pulp injury. SC-specific markers expressed in rat tooth germs at different developmental stages. Nimodipine treatment enhanced mRNA levels of osteogenic markers (DSPP, DMP1, and Runx2) but decreased calcium nodule formation. SCs-derived cells increased following pulp injury and Ca1.2 showed a similar response pattern as SCs. The different SCs phenotypes are coordinated in the whole process to ensure tooth development. Blocking the LTCC with nimodipine promoted SCs odontogenic differentiation. Moreover, SCs participate in the process of injured dental pulp repair as a source of MSCs, and Ca1.2 may regulate this process.
施万细胞（SCs）是外周神经系统中的一种神经胶质细胞，可作为间充质干细胞（MSCs）的来源用于修复损伤的牙髓。本研究旨在探讨施万细胞在牙胚发育和牙髓损伤修复中的作用。我们对不同发育阶段的牙胚进行了RNA测序和免疫荧光染色。通过实时聚合酶链反应和茜素红S染色分析了L型钙通道（LTCC）阻滞剂尼莫地平对施万细胞成牙分化的影响。我们使用PLP1-CreERT2/Rosa26-GFP追踪小鼠模型来研究施万细胞和Ca1.2在牙髓损伤后自我修复中的作用。施万细胞特异性标志物在大鼠不同发育阶段的牙胚中表达。尼莫地平处理可提高成骨标志物（DSPP、DMP1和Runx2）的mRNA水平，但减少钙结节形成。牙髓损伤后施万细胞来源的细胞增加，且Ca1.2显示出与施万细胞相似的反应模式。不同的施万细胞表型在整个过程中相互协调以确保牙齿发育。用尼莫地平阻断LTCC可促进施万细胞成牙分化。此外，施万细胞作为间充质干细胞的来源参与损伤牙髓的修复过程，且Ca1.2可能调节这一过程。
