Laboratory for Cardiac and Thoracic Diagnosis, Regeneration and Applied Immunology, Department of Thoracic Surgery, Medical University of Vienna, Vienna, Austria.
Aposcience AG (FN 308089y), Dresdner Straße 87/A21, Vienna, Austria.
Exp Mol Med. 2022 Nov;54(11):1886-1900. doi: 10.1038/s12276-022-00874-1. Epub 2022 Nov 4.
Recently, a specific Schwann cell type with profibrotic and tissue regenerative properties that contributes to keloid formation has been identified. In the present study, we reanalyzed published single-cell RNA sequencing (scRNA-seq) studies of keloids, healthy skin, and normal scars to reliably determine the specific gene expression profile of keloid-specific Schwann cell types in more detail. We were able to confirm the presence of the repair-like, profibrotic Schwann cell type in the datasets of all three studies and identified a specific gene-set for these Schwann cells. In contrast to keloids, in normal scars, the number of Schwann cells was not increased, nor was their gene expression profile distinctly different from that of Schwann cells of normal skin. In addition, our bioinformatics analysis provided evidence for a role of transcription factors of the AP1, STAT, and KLF families, and members of the IER genes in the dedifferentiation process of keloidal Schwann cells. Together, our analysis strengthens the role of the profibrotic Schwann cell type in the formation of keloids. Knowledge of the exact gene expression profile of these Schwann cells will facilitate their identification in other organs and diseases.
最近，已经鉴定出一种具有成纤维增生和组织再生特性的特定雪旺细胞类型，它有助于瘢痕疙瘩的形成。在本研究中，我们重新分析了瘢痕疙瘩、健康皮肤和正常瘢痕的已发表的单细胞 RNA 测序 (scRNA-seq) 研究，以更详细地可靠确定瘢痕疙瘩特异性雪旺细胞类型的特定基因表达谱。我们能够确认在所有三个研究的数据集均存在修复样成纤维增生性雪旺细胞类型，并确定了这些雪旺细胞的特定基因集。与瘢痕疙瘩不同，在正常瘢痕中，雪旺细胞的数量没有增加，其基因表达谱也与正常皮肤的雪旺细胞明显不同。此外，我们的生物信息学分析为 AP1、STAT 和 KLF 家族的转录因子以及 IER 基因成员在瘢痕疙瘩雪旺细胞去分化过程中的作用提供了证据。总之，我们的分析加强了成纤维增生性雪旺细胞类型在瘢痕疙瘩形成中的作用。这些雪旺细胞的确切基因表达谱的知识将有助于在其他器官和疾病中识别它们。
