Organometallics and Nanomaterials, Department of Chemical Sciences, University of the Western Cape, Bellville, South Africa.
Department of Science and Innovation (DSI)/Mintek Nanotechnology Innovation Centre (NIC) Biolabels Research Node, Department of Biotechnology, University of the Western Cape, Bellville, South Africa.
Artif Cells Nanomed Biotechnol. 2024 Dec;52(1):399-410. doi: 10.1080/21691401.2024.2383583. Epub 2024 Jul 28.
Gold nanoparticles (AuNPs) were synthesized using three red wine extracts (RW-Es); by varying temperature, pH, concentrations of RW-Es and gold salt. The RW-AuNPs were characterized by UV-vis, transmission electron microscopy (TEM), dynamic light scattering (DLS), and the Fourier Transform Infra-red Spectroscopy (FT-IR). Their stability was evaluated in water, foetal bovine serum (FBS), phosphate-buffered saline (PBS), and Dulbecco's Modified Eagle Medium (DMEM) by UV-Vis. The effect of the RW-Es and RW-AuNPs on KMST-6 cell cell viability was evaluated by MTT assay; and their wound healing effects were monitored by scratch assay. RW-AuNPs synthesis was observed by colour change, and confirmed by UV-Vis spectrum, with an absorption peak around 550 nm. The hydrodynamic sizes of the RW-AuNPs ranged between 10 and 100 nm. Polyphenols, carboxylic acids, and amino acids are some of functional groups in the RW-Es that were involved in the reduction of RW-AuNPs. The RW-AuNPs were stable in test solutions and showed no cytotoxicity to the KMST-6 cells up to 72 h. AuNPs synthesized from Pinotage and Cabernet Sauvignon enhanced proliferation of KMST-6 cells and showed potential as wound healing agents. Further studies are required to investigate the molecular mechanisms involved in the potential wound-healing effect of the RW-AuNPs.
金纳米粒子（AuNPs）是使用三种红葡萄酒提取物（RW-Es）通过改变温度、pH 值、RW-Es 和金盐的浓度来合成的。通过紫外可见光谱、透射电子显微镜（TEM）、动态光散射（DLS）和傅里叶变换红外光谱（FT-IR）对 RW-AuNPs 进行了表征。通过紫外可见光谱评估了它们在水中、胎牛血清（FBS）、磷酸盐缓冲盐水（PBS）和 Dulbecco 改良 Eagle 培养基（DMEM）中的稳定性。通过 MTT 测定法评估了 RW-Es 和 RW-AuNPs 对 KMST-6 细胞活力的影响；并通过划痕试验监测其伤口愈合效果。通过颜色变化观察到 RW-AuNPs 的合成，并通过紫外可见光谱证实，在 550nm 左右有一个吸收峰。RW-AuNPs 的水动力粒径在 10nm 至 100nm 之间。多酚、羧酸和氨基酸是参与 RW-AuNPs 还原的 RW-Es 中的一些功能基团。RW-AuNPs 在测试溶液中稳定，在 72h 内对 KMST-6 细胞没有细胞毒性。由皮诺塔吉和赤霞珠合成的 AuNPs 增强了 KMST-6 细胞的增殖，并显示出作为伤口愈合剂的潜力。需要进一步研究以探讨 RW-AuNPs 潜在的伤口愈合作用所涉及的分子机制。
