International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China.
Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing, Changchun University of Science and Technology, Changchun 130022, China.
Anal Methods. 2024 Aug 15;16(32):5527-5535. doi: 10.1039/d4ay00929k.
This paper presents a method for using atomic force microscopy to probe action potentials of single beating cardiomyocytes at the nanoscale. In this work, the conductive tip of an atomic force microscope (AFM) was used as a nanoelectrode to record the action potentials of self-beating cardiomyocytes in both the non-constant force contact mode and the constant force contact mode. An electrical model of a tip-cell interface was developed and the indentation force effect on the seal of an AFM conductive tip-cell membrane was theoretically analyzed. The force feedback of AFM allowed for the precise control of tip-cell contact, and enabled reliable measurements. The feasibility of simultaneously recording the action potentials and force information during the contraction of the same beating cardiomyocyte was studied. Furthermore, the AFM tip electrode was used to probe the differences of action potentials using different drugs. This method provides a way at the nanoscale for electrophysiological studies on single beating cardiomyocytes, neurons, and ion channels embedded within the cell membrane in relation to disease states, pharmaceutical drug testing and screening.
本文提出了一种利用原子力显微镜在纳米尺度探测单个跳动心肌细胞动作电位的方法。在这项工作中，原子力显微镜（AFM）的导电尖端被用作纳米电极，以记录自跳动心肌细胞在非恒力接触模式和恒力接触模式下的动作电位。建立了针尖-细胞界面的电学模型，并从理论上分析了针尖-细胞膜密封的压痕力效应。原子力显微镜的力反馈允许对针尖-细胞接触进行精确控制，从而实现可靠的测量。研究了在同一跳动心肌细胞收缩过程中同时记录动作电位和力信息的可行性。此外，还使用 AFM 尖端电极研究了使用不同药物时动作电位的差异。该方法为在纳米尺度上研究与疾病状态、药物测试和筛选有关的单个跳动心肌细胞、神经元和嵌入细胞膜中的离子通道的电生理学提供了一种途径。
