Hongo S, Matsumoto T, Suzuki I, Sato T
J Biochem. 1979 Aug;86(2):385-90. doi: 10.1093/oxfordjournals.jbchem.a132537.
The activity of rat liver asparagine synthetase [EC 6.3.1.1]increased when animals maintained on 25% protein diet were placed on 15% or 6% protein diet. The enzyme activity level rose within one day, reached a maximum in 7 or 10 days after switching the diet and thereafter dropped gradually. During the purification of the enzyme from rats on 25% or 6% protein diet, the yield and increase of the specific activity were similar in the two groups. Combination of the liver extracts from two such groups demonstrated that the amount of endogeneous inhibitors of the enzyme did not change on replacing the diet. The elevation of the enzyme activity in rats fed 6% casein diet was suppressed by an injection of cycloheximide or actinomycin D. It is suggested that the change in the enzyme activity was due to alteration of the amount of the enzyme.
当给食用25%蛋白质饮食的大鼠改为喂食15%或6%蛋白质饮食时，大鼠肝脏天冬酰胺合成酶[EC 6.3.1.1]的活性增加。酶活性水平在一天内上升，在改变饮食后7或10天达到最大值，此后逐渐下降。在从食用25%或6%蛋白质饮食的大鼠中纯化该酶的过程中，两组的产率和比活性增加相似。将来自两个这样的组的肝脏提取物混合表明，在更换饮食时酶的内源性抑制剂的量没有变化。注射环己酰亚胺或放线菌素D可抑制喂食6%酪蛋白饮食的大鼠中酶活性的升高。提示酶活性的变化是由于酶量的改变。
