Markin R S, Schuster S M
Metabolism. 1984 Oct;33(10):891-6. doi: 10.1016/0026-0495(84)90241-5.
Rats were fed Roger's synthetic amino acid diets, complete or without asparagine, aspartate, or glutamate, for 2 weeks. Hepatic and serum amino acid content and hepatic asparagine synthetase were assayed before and after the test diets were administered. Special attention was paid to the multiple forms of asparagine synthetase present. Rats fed the test diet without asparagine exhibited high molecular weight (110,000) alpha-form and intermediate molecular weight (57,000) beta-form of asparagine synthetase. Rats fed the complete test diet and the test diet without asparatate exhibited only the beta-form of the enzyme. Rats fed the test diet without glutamate exhibited no enzyme activity. These data are related to the hepatic amino acid levels and to the role of asparagine and asparagine synthetase in amino acid homeostasis.
将大鼠喂食罗杰氏合成氨基酸日粮（完整日粮或不含天冬酰胺、天冬氨酸或谷氨酸的日粮），持续2周。在给予试验日粮之前和之后，测定肝脏和血清中的氨基酸含量以及肝脏天冬酰胺合成酶。特别关注所存在的天冬酰胺合成酶的多种形式。喂食不含天冬酰胺试验日粮的大鼠表现出高分子量（110,000）的α型和中等分子量（57,000）的β型天冬酰胺合成酶。喂食完整试验日粮和不含天冬氨酸试验日粮的大鼠仅表现出该酶的β型。喂食不含谷氨酸试验日粮的大鼠未表现出酶活性。这些数据与肝脏氨基酸水平以及天冬酰胺和天冬酰胺合成酶在氨基酸稳态中的作用有关。
