GigaByte. 2024 Jul 18;2024:1-13. doi: 10.46471/gigabyte.130. eCollection 2024.
, or black-faced spoonbill (Threskiornithidae), is a wading bird confined to coastal areas in East Asia. Due to habitat destruction, it was classified as globally endangered by the International Union for Conservation of Nature. However, the lack of genomic resources for this species hinders the understanding of its biology and diversity, and the development of conservation measures. Here, we report the first chromosomal-level genome assembly of using a combination of PacBio SMRT and Omni-C scaffolding technologies. The assembled genome (1.24 Gb) contains 95.33% of the sequences anchored to 31 pseudomolecules. The genome assembly has high sequence continuity with scaffold length N50 = 53 Mb. We predicted 18,780 protein-coding genes and measured high BUSCO score completeness (97.3%). Finally, we revealed 6,155,417 bi-allelic single nucleotide polymorphisms, accounting for ∼5% of the genome. This resource offers new opportunities for studying the black-faced spoonbill and developing conservation measures for this species.
黑脸琵鹭（鹮科）是一种仅限于东亚沿海地区的涉禽。由于栖息地遭到破坏，它被国际自然保护联盟列为全球濒危物种。然而，该物种缺乏基因组资源，这阻碍了对其生物学特性和多样性的了解，以及保护措施的制定。在此，我们报告了首次使用PacBio SMRT和Omni-C支架技术相结合对黑脸琵鹭进行的染色体水平基因组组装。组装后的基因组（1.24Gb）包含95.33%锚定到31条假分子上的序列。该基因组组装具有高序列连续性，支架长度N50 = 53Mb。我们预测了18780个蛋白质编码基因，并测得较高的BUSCO评分完整性（97.3%）。最后，我们揭示了6155417个双等位基因单核苷酸多态性，约占基因组的5%。这一资源为研究黑脸琵鹭和制定该物种的保护措施提供了新的机会。
