GigaByte. 2024 Apr 25;2024:gigabyte122. doi: 10.46471/gigabyte.122. eCollection 2024.
The golden birdwing (Lepidoptera, Papilionidae), a significant species in Asia, faces habitat loss due to urbanization and human activities, necessitating its protection. However, the lack of genomic resources hinders our understanding of their biology and diversity, and impedes our conservation efforts based on genetic information or markers. Here, we present the first chromosomal-level genome assembly of using PacBio SMRT and Omni-C scaffolding technologies. The assembled genome (351 Mb) contains 98.94% of the sequences anchored to 30 pseudo-molecules. The genome assembly has high sequence continuity with contig length N50 = 11.67 Mb and L50 = 14, and scaffold length N50 = 12.2 Mb and L50 = 13. A total of 24,946 protein-coding genes were predicted, with high BUSCO score completeness (98.8% and 94.7% of genome and proteome BUSCO, respectively. This genome offers a significant resource for understanding the swallowtail butterfly biology and carrying out its conservation.
金鸟翼凤蝶(鳞翅目,凤蝶科)是亚洲的一个重要物种,由于城市化和人类活动而面临栖息地丧失,因此需要对其进行保护。然而,缺乏基因组资源阻碍了我们对它们生物学特性和多样性的了解,也妨碍了我们基于遗传信息或标记开展的保护工作。在此,我们利用PacBio SMRT和Omni-C支架技术首次展示了金鸟翼凤蝶的染色体水平基因组组装。组装后的基因组(351 Mb)包含98.94%锚定到30条假分子上的序列。该基因组组装具有高序列连续性,重叠群长度N50 = 11.67 Mb,L50 = 14,支架长度N50 = 12.2 Mb,L50 = 13。总共预测了24,946个蛋白质编码基因,BUSCO评分完整性高(基因组和蛋白质组BUSCO分别为98.8%和94.7%)。该基因组为了解燕尾蝶生物学特性和开展其保护工作提供了重要资源。