Xu Yiming, Wang Wanqiu, Huang Jiefeng, Xu Minjie, Wang Binhu, Wu Yingsong, Xie Yongzhong, Jian Jianbo
Animal Husbandry and Aquatic Affairs Center, Lianyuan City 417100, Hunan Province, China.
BGI Genomics, BGI Center, 9 Yunhua Road, Yantian District, Shenzhen, 518081, China.
GigaByte. 2024 Jul 18;2024:1-15. doi: 10.46471/gigabyte.131. eCollection 2024.
Kinship and pedigree, used for estimating inbreeding, heritability, selection, and gene flow, are useful for breeding and animal conservation. However, as the size of crossbred populations increases, inaccurate generation and parentage assignment in livestock farms increase. Restriction-site-associated DNA sequencing is a cost-effective platform for single nucleotide polymorphism (SNP) discovery and genotyping. Here, we performed a kinship analysis and pedigree reconstruction for Angus and Xiangxi yellow cattle. A total of 975 cattle, including 923 offspring with 24 known sires and 28 known dams, were sampled and subjected to SNP discovery and genotyping. The identified SNP panel included 7,305 SNPs capturing the maximum difference between paternal and maternal genome information, allowing us to distinguish F1 from F2 generations with 90% accuracy. In conclusion, we provided a low-cost and efficient SNP panel for kinship analyses and the improvement of local genetic resources, which are valuable for breed improvement, local resource utilization, and conservation.
亲缘关系和系谱可用于估计近亲繁殖、遗传力、选择和基因流动，对育种和动物保护很有用。然而，随着杂交种群规模的增加，养殖场中世代和亲子关系分配不准确的情况也在增加。限制性内切酶相关DNA测序是一种用于单核苷酸多态性（SNP）发现和基因分型的经济高效平台。在此，我们对安格斯牛和湘西黄牛进行了亲缘关系分析和系谱重建。共采集了975头牛的样本，其中包括923头后代以及24头已知父本和28头已知母本，并对其进行SNP发现和基因分型。所鉴定的SNP面板包含7305个SNP，能够捕捉父本和母本基因组信息之间的最大差异，使我们能够以90%的准确率区分F1代和F2代。总之，我们提供了一个低成本且高效的SNP面板用于亲缘关系分析和地方遗传资源的改良，这对于品种改良、地方资源利用和保护具有重要价值。
