Effect of natural silk fibers and synthetic fiber-reinforced composites on the cytotoxicity of fibroblast cell lines.

BACKGROUND

Synthetic fibers have many benefits in clinical practice; however, they cause microplastic pollution, and their unaffordable price increases treatment costs. Natural silk fibers require biocompatibility assessment. This study investigated the effects of natural and synthetic fiber-reinforced composites (FRCs) on the cytotoxicity of fibroblast cell lines.

METHODS

Three commercial synthetic fibers (polyethylene, quartz, and E-glass) and two silk fibers from and cocoons were employed. These fibers were made into FRC samples (n=6) by impregnation in flowable composite using a brass mold (25×2×2 mm). NIH/3T3 mouse fibroblasts were cultured in Dulbecco's modified eagle medium, supplemented, and seeded in 2×10 cells/mL. They were stored at 37 °C under 5% CO for 24 hours. The FRC samples were made into powder, eluted in dimethylsulfoxide, continued with PBS, supplemented with Dulbecco's modified eagle medium (DMEM), and exposed to cells for 24 hours. Blank (medium only) and control (cells and medium) samples were included. Subsequently, MTT was added for 4 h and read by enzyme-linked immunosorbent assay (λ=570 nm). Cell viability (%) was calculated and analyzed using one-way ANOVA (α=0.05).

RESULTS

All groups of FRCs showed>80% cell viability. One-way ANOVA showed no significant difference between FRC groups regarding the viability of fibroblast cell lines (>0.05).

CONCLUSION

Both natural silk and synthetic fibers exhibit low cytotoxicity to fibroblast cell lines. and silk fibers showed the potential to be used as alternative synthetic fibers.