Klein-Junior Celso Afonso, Zimmer Roberto, Dobler Tãnyre, Oliveira Vanessa, Marinowic Daniel Rodrigo, Özkömür Ahmet, Reston Eduardo Galia
Department of Operative Dentistry, School of Dentistry, Lutheran University of Brazil, Canoas, RS, Brazil.
Department of Operative Dentistry, School of Dentistry, Lutheran University of Brazil, Cachoeira do Sul, RS, Brazil.
J Dent Res Dent Clin Dent Prospects. 2021 Summer;15(3):152-156. doi: 10.34172/joddd.2021.026. Epub 2021 Aug 25.
Direct pulp capping is a method designed to preserve the exposed dental pulp. Due to good biological, physical, and mechanical properties, new versions of calcium silicate-based materials have been developed as pulp capping materials. The present study aimed to evaluate the cytotoxic effects of four calcium silicate-based pulp capping materials, of which the Bio-C Repair Íon+ is still in an experimental phase. Biodentine, MTA Repair HP, Bio-C Repair, and Bio-C Repair Íon+ cements were dispensed in a metallic matrix to produce 125-mm specimens, which were immersed in Dulbecco's Modified Eagle Medium (DMEM) to obtain extracts. NIH 3T3 cells were cultured and exposed to the extracts for 24 hours and seven days. Cell viability was assessed by the test (MTT). The mean values for the experimental and control groups (without treatment) were compared by analysis of variance (ANOVA) and post hoc Tukey tests, considering a significance level of 5%. All the tested materials demonstrated a reduction in cell viability ( < 0.05). According to ISO 10993-5: 2009 (E), Bio-C Repair Íon+ exhibited mild and moderate cytotoxicity in the 24- hour and 7-day analyses, respectively. Bio-C Repair and Biodentine showed mild cytotoxicity, and MTA Repair HP exhibited moderate cytotoxicity at both intervals. The highest cell viability was demonstrated by Biodentine, MTA, and Repair HP, in descending order. Bio-C Repair and Bio-C Repair Íon+ showed moderate cytotoxicity, similar to MTA Repair HP in the 7-day analysis.
直接盖髓术是一种旨在保存暴露牙髓的方法。由于具有良好的生物学、物理和机械性能，新型硅酸钙基材料已被开发用作盖髓材料。本研究旨在评估四种硅酸钙基盖髓材料的细胞毒性作用，其中Bio-C Repair Íon+仍处于实验阶段。将Biodentine、MTA Repair HP、Bio-C Repair和Bio-C Repair Íon+水泥置于金属基质中制成125毫米的样本，将其浸入杜氏改良 Eagle培养基（DMEM）中以获得提取物。培养NIH 3T3细胞并使其暴露于提取物中24小时和7天。通过MTT试验评估细胞活力。通过方差分析（ANOVA）和事后Tukey检验比较实验组和对照组（未处理）的平均值，显著性水平为5%。所有测试材料均显示细胞活力降低（P<0.05）。根据ISO 10993-5:2009（E），Bio-C Repair Íon+在24小时和7天的分析中分别表现出轻度和中度细胞毒性。Bio-C Repair和Biodentine显示出轻度细胞毒性，MTA Repair HP在两个时间间隔均表现出中度细胞毒性。细胞活力最高的是Biodentine、MTA和Repair HP，顺序依次递减。Bio-C Repair和Bio-C Repair Íon+显示出中度细胞毒性，在7天的分析中与MTA Repair HP相似。
