Nosik D N, Korsun N S, Novokhatskiĭ A S
Vopr Virusol. 1985 Sep-Oct;30(5):600-2.
A comparative study of testing methods for polyclonal and monoclonal antibodies to human interferon using direct and reverse neutralization of the antiviral activity of interferon as well as ELISA was carried out. The activity of antibodies in ELISA was dozens of times higher than in neutralization tests. Polyclonal antibodies from the sera of mice immunized with alpha 2 interferon had a higher neutralizing capacity. M-5 monoclonal antibodies in specimens of ascitic fluid induced by inoculation of mice with hybrid cells exhibited an increase in both binding and neutralizing activity as compared with specimens of the culture fluid. Immunoglobulins from the ascitic and culture fluid of nonproductive myeloma cells as well as hybridomas producing monoclonal antibodies of other specificities showed practically no reaction with interferon in any of the tests under study. The screening of monoclonal antibodies intended for research and biotechnological purposes requires a composite analysis in both neutralization and binding tests in order to recover purposefully the hybrid clones producing antibodies with both or one of these properties.
采用干扰素抗病毒活性的直接中和法和反向中和法以及酶联免疫吸附测定法（ELISA），对人干扰素多克隆抗体和单克隆抗体的检测方法进行了比较研究。ELISA中抗体的活性比中和试验中的活性高几十倍。用α2干扰素免疫的小鼠血清中的多克隆抗体具有更高的中和能力。与培养液标本相比，接种杂交细胞诱导的小鼠腹水中的M-5单克隆抗体在结合活性和中和活性方面均有所增加。在任何一项研究的试验中，非分泌型骨髓瘤细胞以及产生其他特异性单克隆抗体的杂交瘤的腹水和培养液中的免疫球蛋白与干扰素几乎没有反应。为研究和生物技术目的而筛选单克隆抗体需要在中和试验和结合试验中进行综合分析，以便有目的地筛选出产生具有这两种或其中一种特性抗体的杂交克隆。
