Amrutesh Reddy Alluru, Gopi Chander Naveen, Ramesh Reddy Jetti, Muthukumar Balasubramanian
Resident (Prosthodontics), SRM Dental College, Chennai, India.
Professor (Prosthodontics), SRM Dental College, Chennai, India.
Med J Armed Forces India. 2024 Jul-Aug;80(4):428-432. doi: 10.1016/j.mjafi.2022.08.014. Epub 2022 Oct 14.
Biomarkers can aid in determining the success of complete denture. Fewer studies evaluated the biomarkers in edentulous patients. This can aid in planning of better qualitative care for edentulous patients. The study compared the quantities of nitric oxide (NO), salivary alpha amylase (SAA) and thiobarbituric acid reactive substances (TBARS) before and after denture insertion in complete denture patients.
The study involved 25 edentulous patients. The saliva samples were obtained from patients prior, 1 h, and 1 week after denture insertion. Standardized procedure was followed in sample management, transportation and storing. The investigations varied with the biomarkers. SAA was estimated by enzymatic assay. TBARS by lipid peroxidation and NO was assessed by Griess reaction. The obtained data were tabled and statistically analyzed.
The results displayed variation in the level of markers between before and after denture insertion. The mean levels of SAA decreased from 8.26 ± 1.32 nm/ml to 3.44 ± 1.25 nm/ml in following denture insertion. The mean of TBARS and NO increased after denture insertion to 14.33 ± 4.72 nm/ml and NO 22.92 ± 5.79 ng/ml. The results were statistically significant with p < 0.05.
The study concluded that denture insertion decreased the levels of psychological stress marker (SAA) and increased the Oxidative stress marker (TBARS, NO).
生物标志物有助于确定全口义齿的成功与否。较少有研究评估无牙患者的生物标志物。这有助于为无牙患者规划更好的定性护理。本研究比较了全口义齿患者义齿插入前后一氧化氮（NO）、唾液α淀粉酶（SAA）和硫代巴比妥酸反应性物质（TBARS）的含量。
本研究纳入25名无牙患者。在义齿插入前、插入后1小时和1周采集患者的唾液样本。样本管理、运输和储存遵循标准化程序。根据生物标志物的不同进行检测。SAA通过酶法测定。TBARS通过脂质过氧化法测定，NO通过格里斯反应评估。将获得的数据制成表格并进行统计分析。
结果显示义齿插入前后标志物水平存在差异。义齿插入后，SAA的平均水平从8.26±1.32纳米/毫升降至3.44±1.25纳米/毫升。义齿插入后，TBARS和NO的平均值分别增加至14.33±4.72纳米/毫升和22.92±5.79纳克/毫升。结果具有统计学意义，p<0.05。
该研究得出结论，义齿插入降低了心理应激标志物（SAA）的水平，增加了氧化应激标志物（TBARS、NO）的水平。
